摘要
目的：采用高效液相色谱法测定雄芍颗粒中芍药苷的含量.方法：采用HPLC法,色谱柱：Diamonsil C18（250 mm×4.6mm,5μm）;流动相：乙腈-0.1％磷酸溶液（14∶ 86）;流速：1.0 mL/min;检测波长：230 nm.结果：芍药苷在0.1078～2.156 0 μg线性关系良好（r=0.999 7）;平均回收率为99.26％,RSD为2.06％（n=5）.结论：该方法简便、准确、可靠、重现性好,可用于雄芍颗粒的质量控制.
Objective： To determine Paeoniflorin in Xiongshao Granule by HPLC. Method： HPLC was used in the quantitative analysis of Paeoniflorin in the sample. The separation was performed on Diamonsil C18 （250 mm× 4.6 mm,5μm） column with a mixture of acetonitrile 0. 1% phosphoric acid （ 14：86） as mobile phase. The flow rate was 1.0 mL/min and the detection wavelength was set at 230 nm. Result： The linear range of paeoniflorin was 0. 107 8 -2. 156 0 μg （ r = 0. 999 7 ）, the average recovery was 99.26%, RSD = 2. 06% （ n = 5 ）. Conclusion： The method is simple, accurate, reliable, good reproductive and could be used for the quality control of paeoniflorin in Xiongshao granule.
出处
《山西中医》
2014年第9期48-49,共2页
Shanxi Journal of Traditional Chinese Medicine
基金
山西省太原市大学生创新创业专题项目(编号:100115176)
关键词
雄芍颗粒
白芍
芍药苷
高效液相色谱法
xiongshao granule, paeonia, lactiflora, paeoniflorin, HPLC
