摘要
目的:比较考马斯亮蓝(Coomassie brilliant blue,CBB)R250和G250两种凝胶染色方法的特点及染色效果。方法:用R250和G250两种染液在同一条件下对完全相同的两块聚丙烯酰胺凝胶进行染色,观察比较其染色及漂洗的时间、效果、灵敏度、安全性等指标。结果:CBB R250染色至少需要2 h,漂洗4 h以上可见蛋白条带;CBB G250染色2 h后不需漂洗即可见蛋白条带,漂洗2 h后背景几乎无色,条带清晰。结论:G250染色在所需时间、效果、灵敏度、安全性和相关度等方面均优于R250,适用于要求快速出结果的重复性好、稳定性高的染色。
Objective：To compare the characteristics and effects of coomassie brilliant blue（CCB）R250 and G250 stai-ning methods. Methods：Two identical polyacrylamide gel were stained by CBB G250 and R250 at the same condition,respec-tively. The time of staining and rinsing,effect,sensitivity and safety were compared. Results：It took at least 2 hours for staining and 4 hours for rinsing to show protein bands using CBB R250. However,the protein bands could be seen after 2 hour staining by CBB G250,and the bands were clear after 2 hour rinsing. Conclusion：The staining effct of CBB G250 is better than that of CBB R250 in sensitivity,dying and rinsing time,security etc. CBB G250 staining method can be applied to fast and stable staining with good reproducibility.
出处
《包头医学院学报》
CAS
2014年第3期1-3,共3页
Journal of Baotou Medical College
基金
国家自然科学基金(81160214)
教育部科学技术研究重点项目(210039)
内蒙古自然科学基金(2010BS1101)
自治区研究生科研创新资助项目(S201310127-Y01)
内蒙古卫生科技计划项目(2010056)
