摘要
目的 分析慢性丙型肝炎患者外周血树突细胞含量与HCV-RNA病毒载量的相关性,探讨HCV感染慢性化的免疫学机制.方法 流式细胞仪检测40例慢性丙型肝炎患者（CHC）外周血中髓样树突细胞（mDC）及浆样树突细胞（pDC）的含量,22例健康人作对照比较.实时荧光定量PCR法检测外周血HCV-RNA载量.结果 HCV-RNA1＋ E003 IU/ml患者及HCV-RNA≥1＋E003 IU/ml患者外周血mDC表达（0.25％±0.09％,0.14％±0.07％）均低于健康对照组（0.38％±0.10％）,差异有统计学意义（t=4.340 9,9.222,P值均＜0.05）;HCV-RNA1＋ E003 IU/ml患者及HCV-RNA≥1＋E003 IU/ml患者外周血pDC（0.23％±0.10％,0.13％±0.08％）均低于健康对照组（0.33％±0.09％）,差异有统计学意义（t=6.552 4,11.04,P值均＜0.05）;HCV RNA≥1＋E003 IU/ml患者外周血mDC,pDC的表达量明显低于HCVRNA1＋E003 IU/ml患者的表达,差异有统计学意义（t=4.349 6,2.671 8,P值均＜0.05）.结论 CHC患者外周血DC含量与HCV-RNA病毒载量呈负相关,CHC患者免疫功能低下.
Objective To analyze the correlation between the proportion of dendritic cells and the levels of viral in peripheral blood of patients with chronic hepatitis C,and to explore the immunological mechanism of chronic HCV infection.Methods The proportion of mDCs and pDCs were tested in peripheral blood from 40 cases of patients with chronic hepatitis C （CHC） by FCM,compared with 22 cases of healthy people as control.The levels of HCV RNA in peripheral blood of patients with CHV were detected by the method of real time fluorescence quantitative PCR.Results HCV-RNA1＋E003 IU/ml patients and HCVRNA≥1＋E003 patients mDC （0.25％±0.09％,0.14％±0.07％） were lower than in the healthy control group （0.38 ％ ± 0.10 ％） （t=4.340 9,9.222,P＜0.05）.HCV RNA1 ＋ E003 IU/ml patients and HCV RNA≥ 1 ＋ E003 patients pDC （0.23％±0.10％,0.13％ ±0.08％）were lower than in the healthy control group （0.33％ ±0.09％）,（t=6.552 4,11.04,P＜0.05）.With the increase of the levels of HCV RNA,the proportion of mDC and pDC decreased gradually （t=4.349 6,2.671 8,P＜0.05）.Conclusion The proportion of dendritic cells was negatively related to the levels of viral in peripheral blood in patients with CHC and immune function is lower in patients with CHC.
出处
《现代检验医学杂志》
CAS
2014年第4期15-16,20,共3页
Journal of Modern Laboratory Medicine
基金
吉林省科技厅发展计划项目,编号20120954.
