摘要
在培养液中加入不同浓度(4.94、9.89μmol/L)的丙二醛(MDA)及酵母培养物水溶物,观察酵母培养物水溶物不同浓度(50、100、200 mg/L)、不同作用时间(3、6、9、12 h)下MDA损伤的离体草鱼肠道黏膜细胞生长、形态及相关酶活力的变化,以研究酵母培养物水溶物对MDA损伤的离体草鱼肠道黏膜细胞的保护作用。结果表明:培养液中添加4.94或9.89μmol/L MDA均显著抑制了离体草鱼肠道黏膜细胞生长(P<0.05),致使贴壁细胞减少、细胞膜通透性增加,导致胞内酶漏出及细胞抗氧化酶活力降低。培养液中添加50、100、200 mg/L酵母培养物水溶物9 h后可显著地促进细胞生长及提高细胞总蛋白含量(P<0.05),改善细胞生长状态,其中以添加100 mg/L酵母培养物水溶物对4.94μmol/L MDA损伤细胞的保护效果较好,其细胞生长状态能达到正常组水平。培养液中添加50、100、200酵母培养物水溶物能一定程度地降低培养液中MDA浓度,同时能降低MDA导致的细胞内酶漏出,提高MDA损伤的细胞抗氧化能力。由结果可知,对于4.94、9.89μmol/L MDA对草鱼肠道黏膜细胞产生的损伤,培养液中添加50、100、200 mg/L酵母培养物水溶物12 h内对细胞均有保护作用,其中以添加100 mg/L酵母培养物水溶物对4.94μmol/L MDA损伤细胞的保护作用最佳,使细胞生长状态接近正常组水平。酵母培养物水溶物可能通过增强细胞抗氧化能力途径起到对草鱼离体肠道黏膜细胞的保护作用。
This experiment was carried out to explore the protective effect of water soluble material of yeast culture on malondialdehyde （MDA） damaged intestinal mucosal cells in vitro of grass carp （ Ctenopharyng- odon idella）. MDA （4.94 and 9.89μmol/L） and water soluble material of yeast culture with different concen- trations were added in culture medium, in order to observe the changes of growth, morphology and relative en- zyme activities of MDA damaged intestinal mucosal cells in vitro of grass carp under the different concentra- tions （50, 100 and 200 mg/L） and different action time （3, 6, 9 and 12 h） of water soluble material of yeast culture. The results showed as follows： added 4.94 or 9.89 μmol/L MDA in culture medium significantly in- hibited the growth of cells （P〈0.05）, caused adherent cells decrease and cellular membrane permeability in- crease, and led to intracellular enzyme leakage and anti-oxidase activities of cells decrease. Cellular growth and cellular total protein content were significantly increased （P〈 0.05 ） and cellular growth conditions were improved on 9 h after water soluble material of yeast culture added in culture medium with the concentrations of 50, 100 and 200 mg/L. Water soluble material of yeast culture with the concentration of 100 mg/L had a better protective effect for cells which damaged by 4.94 μmol/L MDA, and there was no difference in cellular growth conditions compared with normal group. The concentration of MDA and leakage of intracellular enzyme were decreased in a certain extent, and the anti-oxidative ability of cells damaged by MDA was improved when adding 50, 100 and 200 mg/L water soluble material of yeast culture in culture medium. In conclusion, the intestinal mucosal cells in vitro of grass carp damaged by 4.94 and 9.89 μmol/L MDA can be protected by 50, 100 and 200 mg/L water soluble material of yeast culture within 12 h. Adding 100 mg/L water soluble materi- al of yeast culture has the best protective effect for cells which dam
出处
《动物营养学报》
CAS
CSCD
北大核心
2014年第9期2652-2663,共12页
CHINESE JOURNAL OF ANIMAL NUTRITION
基金
国家自然科学基金项目(31172417)
苏州市应用基础(农业)项目(N313401210)
关键词
肠道黏膜细胞
酵母培养物
丙二醛
细胞生长
intestinal mucosal cells
yeast culture
malondialdehyde
cellular growth
