摘要
背景:研究表明骨髓间充质干细胞移植在临床治疗方面具有广阔的应用前景。然而,移植细胞的死亡限制了组织再生,寻找新的抗自由基和保护骨髓间充质干细胞的药物有着重要意义。目的:研究芒果苷对体外培养大鼠骨髓间充质干细胞缺氧损伤的保护作用。方法:采用氯化钴(CoCl2)建立体外培养的大鼠骨髓间充质干细胞缺氧损伤模型。将细胞分为正常对照组、氯化钴缺氧损伤组、氯化钴加芒果苷20,40,80,160μmol/L组。缺氧损伤12,24 h检测各组骨髓间充质干细胞培养上清液中超氧化物歧化酶、丙二醛、过氧化氢酶水平。缺氧损伤3,6,12,24 h检测各组骨髓间充质干细胞内活性氧变化。结果与结论:芒果苷能明显提高缺氧损伤的骨髓间充质干细胞的存活率,提高细胞内超氧化物歧化酶、过氧化氢酶活性,降低细胞内丙二醛、活性氧水平,有效保护缺氧损伤下的骨髓间充质干细胞。芒果苷具有较强的抗氧化能力及缺氧保护作用,可明显减轻骨髓间充质干细胞的氧化应激损伤。
BACKGROUND：Bone marrow mesenchymal stem cells have particularly applied prospects in tissue engineering. However, the death of transplanted cells limits the tissue regeneration. To search a new drug of anti-free radicals and protecting bone marrow mesenchymal stem cells is of great significance. OBJECTIVE：To investigate the protective effects of mangiferin on bone marrow mesenchymal stem cells against hypoxia. METHODS：Rat bone marrow mesenchymal stem cells were cultured in vitro and hypoxia cellmodel was established by cobalt chloride. cells were divided into normal control group, hypoxia group （treated with cobalt chloride）, and mangiferin groups （cobalt chloride＋20, 40, 80, 160 μmol/L mangiferin）. After 12 and 24 hours of hypoxia, superoxide dismutase, malondialdehyde, and catalase levels in the cellsupernatant were determined. After 3, 6, 12, 24 hours of hypoxia, reactive oxygen species change was detected in each group. RESULTS AND CONCLUSION：Mangiferin significantly improved the survival rate of bone marrow mesenchymal stem cells exposed to hypoxia, increased the intracellular superoxide dismutase and catalase activities, decreased intracellular malondialdehyde and reactive oxygen species levels, thereby effectively protecting bone marrow mesenchymal stem cells against hypoxia. These findings indicate that mangiferin has effective protection against hypoxia and strong antioxidant ability, and can significantly reduce oxidative damage.
出处
《中国组织工程研究》
CAS
CSCD
2014年第32期5091-5096,共6页
Chinese Journal of Tissue Engineering Research
基金
广西卫生厅中医药科技专项(GZKZ 10-011)
广西卫生厅中医药科技专项(GZKZ-Z1104)
高等学校博士学科点专项科研基金(20104503110002)
广西科技厅人口健康与食品安全关键技术研究开发项目(桂科攻1140003A-31)
广西卫生厅自筹经费科研课题(桂卫Z2012058
桂卫Z2012061)
广西教育厅立项项目(桂教201010LX050)~~
