摘要
目的对冰冻亚型红细胞制备及使用的全过程进行质量控制,保证其在血型血清学试验中的应用效果。方法对起始血液进行选择,对红细胞甘油化、冰冻保存、融化复温、去甘油化等一系列操作程序进行规范化,对制备好红细胞的保存介质、保存温度以及保存时间进行明确规定并严格执行。结果用优化选择的试验参数制备冰冻亚型红细胞,融化洗涤后红细胞上清液的FHb含量、甘油残留量及红细胞抗原反应性的检测结果均符合预期要求;在规定的有效期内,红细胞性能稳定,使用效果良好。结论对冰冻亚型红细胞制备及使用的全过程进行质量控制,可保证其在工作中的应用效果,提高本地区无偿献血者及临床患者的ABO亚型检出率,保证临床输血的安全性和有效性。
Objective To conduct whole-course quality control over preparation and application of frozen red blood cells to ensure its effect in blood group serology test.Methods Selecting the original blood, conducting standardized procedures including glycerinating, frozen preservation, melting and rewarming, glycerin elimination of the red blood cells, and developing and strictly implementing specific regulations on the preservation medium, temperature and time of the prepared red blood cells.Results The test results of FHb content and residual glycerin in the red blood cell supernatant and antigenic reactivity of red blood cells met expected requirements after preparing frozen red blood cells based on optimized test parameters, and melting and washing red blood cells. The red blood cells demonstrated stable functions and good effects in the period of validity.Conclusion Whole-course quality control over preparation and application of frozen red blood cells can ensure its effects, improve ABO subtype detection rate among voluntary donors and clinical patients in the local area and ensure the safety and effectiveness of clinical transfusion.
出处
《中国医药科学》
2014年第17期136-138,共3页
China Medicine And Pharmacy
基金
2014年度河南省焦作市医学科研项目(2014KY010)
