摘要
目的:观察血管内皮生长因子(vascular endothelial cell growth factor,VEGF)及其受体在糖尿病视网膜病变(diabetic retinopathy,DR)患者玻璃体中的表达,探讨其在DR发生发展中的作用。方法:对13例16眼DR患者和15例15眼健康人,采用免疫组织化学法检测其玻璃体新生血管膜的VEGF及其受体(fms-样酪氨酸激酶,Flt-1和含激酶插入区受体,KDR)表达情况,同时采用酶联免疫吸附法(ELISA)检测DR患者玻璃体中VEGF、Flt-1和KDR的浓度。结果:免疫组织化学染色显示,DR患者玻璃体血管膜中VEGF、Flt-1和KDR高度表达,玻璃体中VEGF、Flt-1和KDR浓度明显高于对照组(P<0.05或P<0.01)。结论:VEGF及其受体Flt-1、KDR在DR患者玻璃体中高度表达,与DR患者的微血管生成关系密切,提示VEGF及其受体在DR的发生发展中起重要作用。
AIM： To investigate the expression of vascular endothelial cell growth factor（ VEGF） and its receptors in vitreous of patients with diabetic retinopathy（ DR）,and to discuss its role in the development of DR.METHODS： Selected 13 patients（ 16 eyes） with DR and15 healthy people（ 15 eyes）,the expression of VEGF and its receptors（ fms-like tyrosine kinase-1,Flt-1 and kinase insert domain containing receptor,KDR） were evaluated by immunohistochemistry in vitreous. The levels of VEGF,the Flt- 1 and KDR in vitreous of patients with DR were examined with enzyme linked immunosorbent assay（ ELISA）.RESULTS： Immunohistochemical staining showed that the expression of VEGF,Flt-1 and KDR in vitreous vessel membrane of patients with DR was increased significantly.And the levels of VEGF,Flt- 1 and KDR in vitreous of patients with DR were obviously higher than that in the control group（ P〈0.05 or P〈0.01）.CONCLUSION： VEGF,Flt- 1 and KDR were widely expressed in vitreous of patients with DR, and were positively related to micro-angiogenesis of DR patients. It proved that VEGF and its receptors played important roles in the occurrence and development of DR.
出处
《国际眼科杂志》
CAS
2014年第9期1587-1589,共3页
International Eye Science
基金
深圳市福田区卫生公益性科研项目(No.FTWS201366)~~
