摘要
目的探讨中药黄芪多糖的体外抗人乳腺癌MCF．7细胞活性。方法实验分为空白对照组、黄芪多糖组和阳性对照组，黄芪多糖组MCF．7细胞给予不同浓度（2．5、5、10、20mg／L）的黄芪多糖，阳性对照组给予10gmol／L顺铂，空白对照组给予等体积培养基。48h后应用四甲基偶氮唑蓝（MTT）法测黄芪多糖对MCF-7细胞增殖抑制率，计算IC_50；吖啶橙（AO），溴化乙啶（EB）荧光染色法测定黄芪多糖对MCF-7细胞诱导凋亡作用；应用流式细胞仪分析黄芪多糖对MCF-7细胞凋亡和细胞周期的影响。结果在给予2．5、5、10、20mg／L黄芪多糖48h后，黄芪多糖呈浓度依赖性抑制MCF．7细胞的增殖（r=0．985，P〈0．05），抑制率分别为（4．14±2．96）％、（7．14±2．10）％、（20．13±2．33）％、（64．66±5．15）％，高于空白对照组0％，但4个不同浓度组的抑制率均低于阳性对照组（90．31±4．92）％。黄芪多糖48h的IC_50=16．83mg／L。随着黄芪多糖浓度的逐渐增高，MCF-7细胞中代表凋亡的玛瑙色逐渐增多，细胞核骤缩、核分裂，细胞形态呈现典型的凋亡特征。2．5、5、10、20mg／L黄芪多糖的凋亡率分别为（2．37±0．98）％、（6．76±1．31）％、（11．65±1．46）％、（20．75±2．68）％，高于空白对照组（1．14±1．25）％（均P〈0．05），但均低于阳性对照组（35．09±2．88）％（均P〈0．05）。与空白对照组比较，黄芪多糖以浓度依赖性诱导MCF-7细胞凋亡（r=0．991，P〈0．05），随浓度的增加，细胞凋亡率升高，但均低于阳性对照组。黄芪多糖随浓度的增加促使s期细胞比例逐渐升高，但低于空白和阳性对照组（均P〈0．05），使处于G_0-G_1期细胞的比例逐渐减少，仍高于空白和阳性对照组（均P〈0．05）。结论黄芪多糖抑制人乳腺癌MCF-7细胞增殖并诱导其凋亡，使MCF-7细胞生长增殖停滞在S期�
Objective To explore the in vitro anti-breast cancer activity of a traditional Chinese medicine, Astragalus polysaccharides （APS）, in MCF-7 ceils. Methods The MCF-7 cells were divided into three groups： treated with different concentrations of APS （2.5, 5, 10 and 20 mg/L） （APS group）, with 10 μmol]L cisplastin （positive control group） , with equivalent volume of media（blank control group）. After 48 h, methylthiazolyl tetrazolium （MTT）test was used to evaluate the proliferation inhibition and then the IC_50 was calculated; acridine orange （AO）-ethidium bromide （EB） fluorescence staining was used to evaluate the induced apoptosis of MCF-7 cells; ftow cytometry was used to analyze the apoptosis and cell cycle. Results After 48 h treatment, different concentrations of APS （2.5, 5, 10 and 20 mg/L） were shown to inhibit the proliferation of MCF-7 cells in a dose-dependent manner （r=0.985, P〈0.05） , with the rates of inhibition being （4.14±2.96）%, （7.14±2.10）%, （20.13±2.33）% and （64.66±5.15）%, respectively. These inhibitionrates were significantly higher than that in blank control group （0%）, but lower than that in positive control group （90.31±4.92）%. The IC_50 of APS at 48 h was 16.83 mg/L. Along with higher concentrations of APS, the MCF- 7 cells showed more apoptosis as reflected by an increase in brown- stained cells and typical morphology of apoptosis such as karyopyknosis. The rates of apoptosis by APS were （2.37±0.98）%, （6.76±1.31）%, （I1.65±1.46）%, （20.75±2.68）%, respectively, according different levels（2.5, 5, 10 and 20 mg/ L）. These rates were higher than that in blank control group （1.14± 1.25 ）%, but lower than that in positive control group （35.09±2.88） % （all P〈0.05）. Compared with blank control group, APS induced apoptosis of MCF-7 cells in a dose-dependent manner （r=0.991, P〈0.05）. The rates of apoptosis increased with higher doses of APS, but were all lower than that in the positive co
出处
《中华生物医学工程杂志》
CAS
2014年第2期106-109,共4页
Chinese Journal of Biomedical Engineering
基金
国家自然科学基金（81172503）
关键词
黄芪多糖
乳腺肿瘤
细胞增殖
细胞凋亡
Astragalan
Breast neoplasms
Cell proliferation
Apoptosis
