摘要
目的 探讨心肌缺血后大鼠孤束核内利钠肽受体NPR-A和NPR-C表达的时程变化,并检测其与胆碱能和儿茶酚胺能神经元之间的关系.方法 雄性SD大鼠分为对照组、假手术组和模型组.复制心肌缺血模型,并于术后的3、7、14、18和28 d取材;用Western blot检测孤束核区域NPR-A和NPR-C的表达量;免疫荧光化学法观察NPR-A、NPR-C与ChAT、TH在孤束核内的共表达.结果 在缺血后14 d孤束核区NPR-A的表达显著低于对照组（P＜0.05）,而后逐渐升高,至缺血后28 d显著高于对照组（P＜0.01）;NPR-C的表达从缺血第3～18天均显著低于对照组（P＜0.01）;孤束核内仅见少量的NPR-A/TH双标记神经元,而NPR-C/TH则较多;未见ChAT与NPR-A或NPR-C双标记神经元的分布.结论 心肌缺血后,在孤束核内利钠肽的调节作用增强,这种调节可能不是直接影响胆碱能和儿茶酚胺能神经元的活动来实现的.
Objective To evaluate time curves of the expression of natriuretic peptide receptor A （NPR-A） and C （NPR-C） in nucleus of the solitary tract after myocardial ischemia （MI） in the rat, and its relation with the cholinergic neurons and the catecholamine neurons. Methods All adult male SD rats were divided randomly into blank control group, sham group and myocardial ischemia （MI） group. After reproduction of the MI model, the rats were sacrificed on day 3, day 7, day 14, day 18 and on day 28 later, and the expression of NPR-A and NPR- C in the area of nucleus of the solitary tract were detected respectively by Western blotting method, and the double labeling of NPR-A, NPR-C with CHAT, TH in the nucleus of the solitary tract was investigated with immunofluorescence method. Results The expression quantity of NPR-A in nucleus of the solitary tract of rat was significantly decreased（P 〈0. 05 ） on day 14, and then increased, until the day 28 later the expression of NPR-A was signifi- cantly increased than blank control group（P 〈 0. 01 ）. While the expression of NPR-C decreased than blank control group on day 3, day 7, day 14 and day 18（P 〈0.01 ）. The immunofluorescence staining detection in the nucleus of the solitary tract indicated that there were many double labeling neurons of NPR-C/TH, but there was a little double labeling neurons of NPR-A/TH, and there has no double labeling neuron of NPR-A, NPR-C with CHAT. Conclusions The regulating action of the natriuretic pepitde on cardiovascular function in the nucleus of the solita- ry tract was enhanced, and this effect is not dependent on the influence with the cholinergic neurons and the cate- cholamine neurons.
出处
《基础医学与临床》
CSCD
北大核心
2014年第9期1226-1230,共5页
Basic and Clinical Medicine
基金
河北省卫生厅医学科学研究重点课题(20090044)
关键词
心肌缺血
利钠肽受体
孤束核
蛋白质印迹
免疫荧光组织化学
大鼠
myocardial ischemia
natriuretic peptide receptor
nucleus of solitary tract
Western blot
immunofluorescence chemistry staining
rats
