摘要
目的：建立蒙药清血八味散中异土木香内酯和土木香内酯含量的方法。方法：采用高效液相色谱法,使用色谱柱为Diamonsil C18（250 mm×4.6 mm,5μm）,流动相为乙腈-0.04%磷酸水溶液（60∶40）,流速为1.0 mL·min-1,检测波长为194 nm,柱温为30℃。结果：异土木香内酯进样量在0.05~0.25μg、土木香内酯进样量在0.03~0.15μg范围内与峰面积具有良好的线性关系,平均回收率为异土木香内酯98.9%,RSD 1.65%;土木香内酯99%,RSD 0.62%。结论：该方法简便、快捷、准确、重复性好,可用于作为清血八味散中异土木香内酯和土木香内酯的含量测定。
Objective ： To establish a method for the determination of isoalantolactone and alantolactone in Bawei Qingxue power by HPLC. Method ： We determined the isoalantolactone and alantolactone in Bawei Qingxue power by HPLC on the chromatographic column of Diamonsil Cla （ 250 mm × 4.6 mm, 5 μm ） and with Acetonitrile-0.4% phosphoric acid （ 60 ： 40, v/v ） as a mobile phase and the flow rate of 1.0 mL · min-1. The detective wavelength was 194 nm, and the temperature of the column was 30 ℃ . Result ： The obvious linear relationship was between the input mass of isoalantolactone and alantolactone as reference substance ranging from 0.05 μg to 0.25 μg and from 0.03 μ g to 0.15 μ g. The average recovery of isoalantolactone was 98.9%, RSD 1.65% and the average recovery of alantolactone was 99%, RSD 0.62%. Conclusion ： The method is simple, rapid, accurate and possesses good reproducibility. It can be used for determining the content of isoalantolactone and alantolactone in Mongolian medicine Bawei Qingxue power.
出处
《辽宁中医药大学学报》
CAS
2014年第9期80-82,共3页
Journal of Liaoning University of Traditional Chinese Medicine
基金
内蒙古民族大学自然科学研究项目(MDX2008046)
