摘要
研究以玉米秸秆源450℃无氧条件制备的生物黑炭为试验材料,设5个处理,即对照(CK,0%)、化肥处理(F,0%生物黑炭+常规施肥,N 100 mg·kg-1、P 100 mg·kg-1、K 150 mg·kg-1)、低量生物黑炭与化肥配施(1%BF,1%生物黑炭+常规施肥)、中等生物黑炭与化肥配施(5%BF,5%生物黑炭+常规施肥)和高量生物黑炭与化肥配施(10%BF,10%生物黑炭+常规施肥)(质量分数)等,研究生物黑炭与化肥配施对大豆根际氮素转化过程中相关功能菌数量、pH及速效氮含量的影响。结果表明,生物黑炭与化肥配施显著增加大豆根际pH;生物黑炭与化肥配施对根际固氮菌数量、氨化细菌数量和硝化细菌数量的影响具有一定剂量效应,5%BF处理对大豆根际氮素转化相关细菌数量、速效氮含量的提升最为有利。为揭示生物黑炭影响养分过程中的微生物机制提供理论依据。
Biochar made by maize straw at 450 ℃ insulated from oxygen was set as materials. Five experimental treatments were as follows：Control with no fertilizer and biochar application, fertilizer treatment （N 100 mg·kg-1, P 100 mg·kg-1, K 150 mg·kg-1 with no biochar application）, fertilizer with few biochar （N 100 mg·kg-1, P 100 mg·kg-1, K 150 mg·kg-1 with 1%biochar）, fertilizer with moderate biochar （N 100 mg·kg-1, P 100 mg·kg-1, K 150 mg·kg-1 with 5%biochar） and fertilizer with mass biochar （N 100 mg·kg-1, P 100 mg·kg-1, K 150 mg·kg-1 with 10%biochar）. The present study was proceeded to analyze the amounts of the functional bacteria, pH and available nitrogen related to nitrogen transformation of rhizosphere in soybean. The results showed that pH of rhizosphere increased significantly, and dosage effect was observed in amount of azotobacteria, ammonification bacteria and nitrobacteria when fertilizer plus biochar was applied. Moreover, 5% biochar application was favorable to increase the amount of bacteria related to nitrogen transformation in soybean rhizosphere. The results above can offer the evidence to reveal the microbial mechanism of biochar effect on the nutritive substance in soybean rhizosphere.
出处
《东北农业大学学报》
CAS
CSCD
北大核心
2014年第8期11-19,26,共10页
Journal of Northeast Agricultural University
基金
国家自然基金(41301316)
黑龙江省博士后科研启动金(LBH-Q13028)
土壤与农业可持续发展重点实验室开放基金(Y412201418)
