摘要
采用不同检测技术(荧光偏振法、中性红法、氧电极法等)测定梢枯病菌蛋白毒素对不同杂交竹品种嫩枝离体线粒体膜的流动性、表面电位、肿胀度、聚集度及脂质过氧化物MDA、辅酶Q10(CoQ10)含量、线粒体呼吸功能的影响。结果表明:蛋白毒素胁迫使4个竹品种的线粒体膜流动性减弱、表面电位减低、肿胀度增大、聚集度降低;CoQ10含量下降,MDA含量增加,细胞色素C氧化酶(CCO)、ATPase活性亦下降,呼吸控制率(RCR)和磷氧比(P/O)明显降低。说明蛋白毒素对杂交竹嫩枝线粒体膜造成损伤,线粒体脂质过氧化程度增加,膜完整性被破坏,呼吸作用受抑制。以上各项指标中,抗病品种(3#和6#)受蛋白毒素的影响均显著低于感病品种(8#和30#)。
Effects of the protein toxin from Arthrinium phaeospermum on the membrane fluidity,surface electronic potential,swelling,aggregation and the contents of lipid peroxide MDA,Coenzyme Q 10 （ CoQ10 ） ,and respiration function of the mitochondria from shoots of different Bambusa pervariabilis ＆#215; Dendrocalamopsis grandisvarieties were determined by the different detection techniques （ fluorescence polarization,neutral red dye,oxygen electrode,etc. ） . The results showed that the protein toxin caused the membrane fluidity weakening,surface electronic potential and aggregation decrease,swelling increase of mitochondria in four bamboo varieties. With the protein toxin treatment,the CoQ10 content decreased,the MDA content increase,the activities of cytochrome c oxidase （ CCO） and ATP decrease, respiratory control ratio （ RCR ） and oxidative phosphorylation ratio （ P/O ） significantly reduced. The above results demonstrated that the protein toxin damaged the mitochondrial membrane, increased the lipid peroxidation of mitochondrion,destroyed the membrane integrality,and inhibited the respiration. In term of all of the above indices,the effect of protein toxin on the resistant varieties （3# and 6#） was significantly weaker than that on the susceptible varieties （8# and 30#）.
出处
《林业科学》
EI
CAS
CSCD
北大核心
2014年第8期119-125,共7页
Scientia Silvae Sinicae
基金
国家自然科学基金项目(31070578)
关键词
蛋白毒素
线粒体
生物物理特性
呼吸作用
protein toxin
mitochondrion
biophysical characteristic
respiration
