摘要
目的探讨miR-126在人结肠癌细胞中的表达及其对结肠癌细胞的耐药性影响。方法采集我院肿瘤科2010年7月至2013年8月收治的45例结肠癌患者的癌组织、癌旁组织及30例同期体检健康对象的正常结肠组织(正常组织),采用荧光PCR检测miR-126表达水平;体外培养HCT116细胞,通过转染抑制序列抑制miR-126的表达,检测抑制HCT116细胞miR-126的表达对结肠癌细胞耐药性的影响。结果结肠癌组织的miR-126阳性表达率显著低于癌旁组织、正常组织(P<0.05);癌旁组织的miR-126阳性表达率低于正常组织但差异不显著(P>0.05);miR-126表达在年龄、性别、分化程度、肿瘤大小间差异不显著(P>0.05),不具统计学意义;miR-126表达与淋巴结转移、Dukes分期、临床分期具有显著的相关性,且miR-126表达水平在各个层之间差异显著(P<0.05);在0、5、10、20 mg/L的奥沙利铂培养基中,miR-126阳性组的癌细胞活性(吸光度值)均显著低于miR-126阴性组且差异具有统计学意义(P<0.05)。结论结肠癌组织中的miR-126阳性表达率较低,癌细胞组织中的miR-126阳性表达有利于降低癌细胞的耐药性,有利于对结肠癌的治疗效果。
Objective To investigate the expression of miR-126 in human colon cancer cells and its ef-fect on drug resistance. Methods Forty five cases of colon cancer tissues, para-carcinoma tissues and 30 cases of normal colon tissues were collected from July 2010 to August 2013 in Department of Oncology of our hos-pital. By detecting miR-126 expression levels using fluorescent PCR, culturing HCT116 cells in vitro, sup-pressing the expression of miR-126 by transfecting the suppressing sequence, we studied the drug resistance effect of miR-126 in HCT116 cells. Results The positive rate of miR-126 expression in colon cancer tissues was significantly lower than that in para-carcinoma tissues and normal tissues （P〈0.05）;moreover, this rate in para-carcinoma tissues was also lower than that in normal tissues, but the difference was not significant （P〉0.05）. No significant differences of miR-126 expression in age, gender, differentiation,and, tumor size were observed （P〉0.05）, but miR-126 expression was found to be associated with lymph node metastasis, Dukes′staging and clinical staging;the differences among miR-126 expression levels was also significant （P〈0.05）. In 0, 5, 10,20 mg/L oxaliplatin medium, cancer cell activity （absorbance） in miR-126 positive group was sig-nificantly lower than in miR-126 negative group （P〈0.05）. Conclusion The positive expression of miR-126 in colon cancer tissues is relatively low, whereas this positive expression can reduce drug resistance of cancer cells and is beneficial to the treatment of colon cancer.
出处
《现代消化及介入诊疗》
2014年第4期226-229,共4页
Modern Interventional Diagnosis and Treatment in Gastroenterology
