摘要
目的:探讨薯蓣丸对变应性鼻炎大鼠模型IL-4、AQP5的影响,并探讨二者之间是否具有相关性。方法:将50只雄性SD大鼠随机分为正常组、模型组、高剂量治疗组、中剂量治疗组、低剂量治疗组,每组各10只。对除正常组外的其余组大鼠采用卵清蛋白(OVA)刺激建立变应性鼻炎大鼠模型,正常组、模型组以生理盐水口饲,高、中、低剂量治疗组用薯蓣丸药液灌胃治疗。测定比较各组大鼠血清IL-4水平以及AQP5表达,并分析二者的相关性。结果:薯蓣丸能改善实验动物鼻炎症状,各治疗组IL-4水平降低、AQP5表达增加,与模型组比较,差异均有统计学意义(P<0.05)。IL-4与AQP5表达呈显著负相关(P<0.05)。结论:薯蓣丸能通过影响IL-4和AQP5缓解变应性鼻炎症状,二者呈显著负相关性。
Objective：To investigate the effects of Dioscorea opposita Thunb. pills on interleukin 4 （IL -4） and aquaporin 5 （AQP5）in a rat model of allergic rhinitis and to analyze their relationship. Methods ： Fifty male rats were randomly divided into normal group, modelgroup, and high -, middle -, and low- dose treatment groups, with 10 rats in each group. Rats were sensitized with ovalbumin to establisha rat model of allergic rhinitis. The normal group and model group were orally fed with normal saline,while the high - ,middle - ,and low - dose treatment groups received Dioscorea opposita Thunb. pills by gavage. After treatment, serum IL - 4 level and AQP5 expression weremeasured. Results ： Dioscorea opposita Thunb. pills relieved the symptoms of allergic rhinitis. The treatment group had a significantly re-duced IL -4 level and a significantly increased AQP5 expression compared with the model group （P 〈 0. 05 ）. IL -4 level showed a signif-icant negative correlation with AQP5 expression （P 〈 0. 05 ）. Conclusion：Dioseorea opposita Thunb. pills can relieve the symptoms of al-lergic rhinitis through IL-4 and AQP5. There is a significant negative correlation between IL- 4 level and AQP5 expression.
出处
《湖南中医杂志》
2014年第8期158-160,共3页
Hunan Journal of Traditional Chinese Medicine
基金
云南省教育厅科学研究基金资助项目(编号:2011Y404)
关键词
变应性鼻炎
薯蓣丸
大鼠
IL-4
AQP5
相关性
allergic rhinitis
Dioscorea opposita Thunb. pill
rat
interleukin 4
aquaporin 5
correlation
