摘要
采用镜检法和荧光定量PCR法对青田县1例输入性疟疾患者血样进行检测。镜检结果显示,患者血样的薄、厚血片中均发现恶性疟原虫,但其原虫密度均较低,为240个/μl血;PCR结果显示,该血样恶性疟原虫特异性DNA片段阳性,确诊为恶性疟原虫感染。
The whole blood sample from a patient with imported falciparum malaria was tested by microscopic examination and fluorescence quantitative PCR. Microscopic examination results showed that Plasmodium falciparum parasites were found in the thick and thin blood films with a low parasite density of 240 parasite/μl of blood. The specific DNA fragment of P. falciparum was amplified by fluorescence quantitative PCR, and this case was further confirmed as P. falciparum infection.
出处
《中国寄生虫学与寄生虫病杂志》
CAS
CSCD
北大核心
2014年第4期316-317,共2页
Chinese Journal of Parasitology and Parasitic Diseases
关键词
输入性疟疾
恶性疟原虫
镜检
荧光定量PCR
诊断
Imported malaria
Plasmodium falciparum
Microscopic examination
Fluorescence quantitative PCR
Diagnosis