摘要
以肺腺癌A549细胞为筛选靶标,人胚肺二倍体helf细胞作反相吸附细胞,从噬菌体随机十二肽库中筛选出与肺癌细胞特异性结合的噬菌体多肽,用其替代一抗,酶标抗噬菌体抗体作二抗,建立检测肺癌细胞的免疫酶染色法。对噬菌体随机十二肽库进行4轮筛选和ELISA鉴定后,用亲和力和特异性最好的5号噬菌体克隆建立了检测肺癌细胞的免疫酶法。
Abstract With A549 cells serving as target cells and helf cells as absorber cells, polypeptides specifically binding to lung cancer ceils were screened out from Ph.D. - 12TM Phage Display Peptide Library. Establish the immu- noenzymatic assay （IEA） with the screened polypeptides replacing primary antibody, and the enzyme-marked anti phage antibody working as secondary antibody. After four rounds of panning and ELISA identification, the phage clone with best affinity and specificity to A549 cells was applied in the new method.
出处
《安徽医科大学学报》
CAS
北大核心
2014年第9期1329-1333,共5页
Acta Universitatis Medicinalis Anhui
基金
安徽省重点科研项目(编号:07021017)
关键词
噬菌体展示肽库
A549细胞
肺癌
免疫酶法
phage display peptide library
A549 cells
applied in the new method lung cancer
immunoenzymetic assay
