摘要
[目的]了解SD大鼠膝关节软骨急性缺损后早期高强度跑台运动对软骨修复的影响。[方法]将24只雄性Sprague-Dawley(SD)大鼠随机分为实验组(A组,n=12)与对照组(B组,n=12),两组大鼠均在双侧股骨内髁制造直径1.6 mm全层关节软骨缺损。建模后两组大鼠分笼饲养,自由活动,A组大鼠每天进行一次定时定量的小动物跑台训练,B组不施加运动干预。建模后2、6、10、14周时两组各随机处死3只大鼠,分别进行实验部位的大体观察和HE染色;然后进行番红O-固绿染色,并采用Image-pro plus 6.0图像分析软件测定单位面积番红O-固绿染色的平均光密度值,用以显示糖氨多糖(glycosaminoglycan,GAG)的含量;参照软骨缺损修复的改良Pineda半定量评分法进行评分。[结果]术后2周时,实验组与对照组大体观察实验部位仍存在缺损凹陷;术后6周时,两组缺损处凹陷已基本填平,表面较光滑,色泽较均匀,术后14周时,两组缺损处已与周围正常软骨无明显区别。术后2周时,HE染色两组均可见缺损区凹陷,有少量肉芽组织填充,间质内毛细血管及炎细胞浸润,少量纤维细胞、肌纤维母细胞增生,番红O-固绿无异染性着色;术后6周时,缺损区内炎症明显减轻,底部可见骨小梁,表层有成纤维细胞覆盖,可见少量岛类软骨细胞生长,修复组织和周边软骨组织之间有裂隙或不连续,缺损边缘的组织细胞少,纤维细胞及肌纤维母细胞明显增生,少量纤维软骨形成,番红O-固绿染色见缺损修复区组织染色明显减少;术后10周时,修复组织与周边软骨组织整合程度增加,主要由透明变形的纤维软骨及类透明软骨细胞填满并延伸到两侧关节软骨面,底部及边缘可见骨组织及软骨增生,番红O-固绿染色见修复组织内染色轻度减少,与正常软骨染色区别不明显;术后14周时,表面主要为纤维软骨细胞或类透明软骨细胞覆盖,底部及边缘软骨增生较前增加,但镜�
[ Objective] To investigate the effects of early high - intensity treadmill exercise on cartilage repair in an animal model of acute articular cartilage defect. [ Method] Twenty - four male Sprague - Dawley （SD） rats were randomly divided into an experimental group （ group A ,n = 12） and a control group （ group B ,n = 12）. A full - thickness cartilage defect model was created,with a diameter of 1.6 mm, on the articular surface of the medial femoral condyles in all animals. Subsequently, the two groups of rats were housed separately,with freedom of movement. The rats in the group A performed treadmill exercise once dai- ly;by contrast,the rats in the group B were left in the cage without any movement intervention. On weeks 2,6,10, and 14 after the operation, three rats in each group were killed and used as samples for the study. The experimental areas of both medial fem- oral condyles were subjected to general observation, and hematoxylin and eosin （ HE ） and safranin - O fast - green staining. Im- age - Pro Plus 6.0 image analysis software was used to measure the unit area of safranin - O fast - areen staining and the meanoptical density of staining for determining the glycosaminoglycan content. Pinedas semiquantitative scale for histologic grading of articular cartilage repair was used for sco- ring. [ Result] On week 2 after the operation, the medial femo- ral condyles remained defective in the experimental group andthe control group during general observation. However,the defect was almost filled, with a smooth surface and uniform color, in both groups on week 6. The articular surface appeared to show no significant difference between the repairing tissue and normal cartilage surface in both groups on week 14. HE staining showed that the defect was still visible and filled with granulation tis- sue, capillaries, and inflammatory cells;a few fibroblasts and myofibroblast proliferation were also observed. Safranin - O fast - green dye were not heterophilic infiltration at this stage on week 2. Th
出处
《中国矫形外科杂志》
CAS
CSCD
北大核心
2014年第16期1492-1497,共6页
Orthopedic Journal of China
基金
南京军区"十一五"计划医学科研重点课题(编号:06Z07)
关键词
SD大鼠
股骨内髁
软骨
跑台运动
组织病理学
SD rat, medial femoral condyle, cartilage, treadmill exercise, histopathology
