摘要
目的探讨局部黏着斑激酶(focal adhesion kinase,FAK)在胃癌组织中的表达意义及与幽门螺杆菌L型(Helicobacter pylori-L,Hp-L)感染的关系。方法 (1)应用免疫组织化学Elivision法检测120例胃癌组织及40例切缘正常胃粘膜组织(对照组)中FAK蛋白的表达情况,采用免疫组织化学和革兰染色法检测Hp-L型的感染情况;(2)采用逆转录多聚酶链反应(RT-PCR)技术检测40例新鲜胃癌组织及对应切缘正常胃黏膜组织(对照组)中FAK的mRNA表达。结果胃癌组FAK蛋白的表达阳性率高于对照组(P<0.05),且FAK的高表达与分化程度、浸润深度、淋巴结转移和TNM分期有关(P<0.05),与年龄、性别、肿瘤大小无关(P>0.05);RT-PCR显示,肿瘤组织、远端正常对照组织的FAK表达量差异明显(P<0.01)。胃癌组Hp-L型检出率72.5%(87/120)与对照组37.5%(15/40)有显著性差异(P<0.05),与免疫组化Hp-L型抗原表达率65.0%(78/120)无显著性差异(P>0.05),Hp-L检出阳性率为69.2%(83/120);胃癌组中Hp-L型感染阳性组的FAK表达阳性率高于Hp-L型阴性组(P<0.05),且Hp-L型阳性率和FAK蛋白的表达呈正相关(r=0.291,P<0.05)。结论FAK蛋白和mRNA在胃癌中的表达增加,且与胃癌的浸润、转移相关,其机制可能与幽门螺杆菌L型(Hp-L型)感染有关。
Objective To investigate the signifcance of focal adhesion kinase（FAK）expression in gas tric carcinoma（GC）and its relationship with the Helicobacter pylori L form （Hp L） infection. Methods （1） Immunohistochemical staining was used to detect the expression of FAK protein in 120 samples of gastric cancer tissue and 40 normal gastric mucosamargin （control group）. Gram＇s and immunohistochemical stai ning were used to detect the Hp L infection. （2） The expression of FAK mRNA in 40 cases of fresh gas tric cancer tissue and the normal gastric mucosa margin （control group） was detected by semi quantitative reverse transcriptase polymerase chain reaction （RT PCR）. Results： The positive rate of FAK protein ex pression in gastric carcinoma group was significantly higher than in the control group （P〈0. 05）, and FAK protein expression increased with differentiation, depth of invasion, lymph node metastasis and TNM stage （P〈0. 05） , but not correlated with patients＇ sex, age and tumor size （P〉0.05）. RT PCR showed significant differences in the FAK expression between tumor and the distal normal control tissues （P〈0.01）. The positive rate of the Hp L form infection （72.5%） in gastric cancer was significantly high er than that in the control group （37.5%） with Gram stain （P〈0.05）. The antigen of Hp Lin 65.0% ca ses was detected by immunohistochemical techniques. There was no difference in the results between the two staining methods （ P〉0. 05）. The positive rate of the infection of Hp L was 69.2% （83/120）.In the gastric cancer group, the expression of FAN was significantly higher in Hp L infection positive cases than that in negative ones （r=0. 291, P〈0.05）. Conclusion The expression of FAK protein and mRNA in gas tric cancer is increased and correlated with tumor＇s invasion and metastasis, and the mechanism may be re lated to Helicobacter pylori L form infection.
出处
《中国组织化学与细胞化学杂志》
CAS
CSCD
2014年第4期325-330,共6页
Chinese Journal of Histochemistry and Cytochemistry
基金
安徽省高校省级自然科学研究重点项目资助(KJ2007A098)
蚌埠市科技局2007年第一批科技项目资助(11)
关键词
胃肿瘤
FAK
幽门螺杆菌
L型
Gastric neoplasm
FAK
Helicobacter Pylori
L form
