摘要
目的探讨HBeAg阴性慢性乙型肝炎(CHB)中医证型与T淋巴细胞亚群及白介素-4(IL-4)、γ干扰素(IFN-γ)之间的相关性。方法对120例HBeAg阴性CHB患者进行辨证分型,选取同期健康人群30例作为对照,检测外周血T淋巴细胞亚群(CD3+、CD4+、CD8+及CD4+/CD8+)及IL-4、IFN-γ水平。结果 HBeAg阴性CHB患者外周血T淋巴细胞亚群与正常对照组比较,CD4+下降,CD8+上升,CD4+/CD8+降低(P<0.05或P<0.01),IL-4水平升高(P<0.01),IFN-γ水平降低(P<0.01);各证型间比较,湿热中阻、瘀血阻络组T淋巴细胞亚群与肝肾阴虚、脾肾阳虚组差异有统计学意义(P<0.05或P<0.01)。按湿热中阻、肝郁脾虚、瘀血阻络、肝肾阴虚、脾肾阳虚组顺序,IL-4水平逐渐上升,IFN-γ水平逐渐下降,相邻两组间差异均具有统计学意义(P<0.01或P<0.05)。结论 HBeAg阴性CHB外周血T淋巴细胞亚群及IL-4、IFN-γ水平变化与中医证型之间有一定相关性,可为HBeAg阴性CHB中医辨证的客观化提供依据。
Objective To evaluate the correlation between TCM dialectical type and T lymphocyte subsets,IL-4,IFN-γ in patients with HBeAg-negative chronic hepatitis B. Methods 120 patients with HBeAg-negative CHB were diagnosed with differential Chinese medicine syndrome,and their peripheral blood T lymphocyte subsets（CD3＋,CD4＋,CD8＋ and CD4＋/CD8＋）,IL-4and IFN-γ level were determined. Results Compared with those in the normal control group,CD4＋ was lower,CD8＋was higher,CD4＋/CD8＋was decreased significantly（P〈0.05 or P〈0.01）,IL-4 was significantly elevated（P〈0.01）and IFN-γ was significantly lower（P〈0.01）in the HBeAg-negative CHB patients. Comparison among the different syndrome types revealed that in damp heat resistance and blood-stasis in meridian,T lymphocyte subsets was statistically different with liver-kidney yin deficiency,spleenkidney yang deficiency（P〈0.05 or P〈0.01）. In the damp heat resistance,liver stagnation and spleen deficiency,blood-stasis in meridian,liver-kidney yin deficiency,spleen-kidney yang deficiency sequence,IL-4 rose gradually and IFN-γ decreased gradually,with significant differences between groups（P〈0.01 or P〈0.05）. Conclusions It is related that the peripheral blood T lymphocyte subsets,IL-4,IFN-γ levels and TCM dialectical type in patients with HBeAg-negative CHB.
出处
《云南中医学院学报》
2014年第4期1-3,8,共4页
Journal of Yunnan University of Traditional Chinese Medicine
基金
国家中医药管理局国家中医临床研究基地业务建设科研专项课题(JDZX2012052)
