摘要
目的：研究雷公藤内酯醇对大鼠脑出血后小胶质细胞、星形胶质细胞的影响.方法：雄性SD大鼠随机分为干预组、对照组和假手术组.采用Ⅳ型胶原酶肝素生理盐水尾状核立体定向注射法建立大鼠脑出血模型,干预组腹膜腔内注射雷公藤内酯醇,连续7d.在术后每日进行1次Longa神经功能缺损评分.采用免疫组织化学检测小胶质细胞和星形胶质细胞.结果：干预组在术后第2、5、7天的Longa神经功能缺损评分低于对照组;干预组术后第2、7天血肿周边活化的小胶质细胞较对照组明显减少;干预组术后第2天星形胶质细胞较对照组明显减少.结论：雷公藤内酯醇可以部分改善大鼠脑出血急性期神经功能,这可能与其抑制小胶质细胞和星形胶质细胞活化,减轻炎症反应有关.
Objective：To explore the effect of triptolide on microglia and astrocyte in intracerebral hemorrhage （ICH） rats.Methods：Male SD rats were randomly divided into an intervention group,a control group and a sham operated group.ICH model was established by microinjecting type Ⅳ collagenase and heparin into rat right caudate nucleus.The intervention group rats were intraperitoneally injected with triptolide everyday for 7 days.Longa neurological defect scores were used for neurological function study on every day after operation.Immunohistochemistry was adopted to assess microglia and astrocyte in the rat brains.Results：Longa neurological defect scores of intervention group were decreased compared with the control group at 2 days,5 days and 7 days after operation; the numbers of microglia and astrocyte were decreased significantly around hematoma in the brains of the intervention group compared with the control group.Conclusion：Triptolide could inhibit activation of microglia and astrocyte and improve partly neurological function in acute ICH rats.
出处
《解剖学杂志》
CAS
CSCD
北大核心
2014年第4期496-499,共4页
Chinese Journal of Anatomy
基金
福建省卫生厅青年科研课题（2010-2-32）
国家临床重点专科项目经费资助
