摘要
采用HPLC法对甜瓜根系分泌物进行分离鉴定,检测到甜瓜根系分泌物中含有没食子酸、邻苯二甲酸、丁香酸、水杨酸、阿魏酸、苯甲酸和肉桂酸7种酚酸物质,通过外源添加法研究该类物质对尖孢镰孢菌的化感效应.室内试验结果表明:阿魏酸、苯甲酸、肉桂酸在0.1、0.25 mmol·L-1处理浓度下能够显著促进尖孢镰孢菌的孢子萌发,水杨酸则对孢子萌发具有一定的抑制作用;丁香酸、阿魏酸在菌丝培养后期表现出较强的促进作用.盆栽结果显示,在0.05、0.1和0.5 mmol·L-1处理浓度下肉桂酸、阿魏酸、苯甲酸可显著促进甜瓜枯萎病病情.
In this study, the phenolic compounds of melon root exudates were identified by HPLC and seven phenolic compounds including gallic acid, phthalic acid, syringic acid, salicylic acid, ferulic acid, benzoic acid and cinnamic acid were observed. The laboratory experiment showed that ferulic acid, benzoic acid and cinnamic acid of 0.1 and 0.25 mmol·L^-1 could significantly pro- mote the germination of Fusarium oxysporum f. sp. melonis spore while salicylic acid inhibited the spore germination to some degree. Syringic acid and ferulic acid significantly promoted the myce- lium growth at the late stage of incubation. The pot experiments demonstrated that cinnamic acid, benzoic acid and ferulic acid enhanced melon infection at concentrations of 0.05, 0.1 and 0.5 mmol·L^-1.
出处
《应用生态学报》
CAS
CSCD
北大核心
2014年第8期2355-2360,共6页
Chinese Journal of Applied Ecology
基金
沈阳市农业科技攻关专项(F12-119-3-00)
国家高技术研究发展计划项目(2006CB101901)
辽宁省科技攻关项目(2011214002)资助
关键词
甜瓜根系分泌物
酚酸物质
化感效应
尖孢镰孢菌
枯萎病
melon root exudates
phenolic compounds
allelopathic effects
Fusarium oxysporumf. sp. melonis
Fusarium wilt.
