摘要
为探究饲料中添加核苷酸对大黄鱼生长性能、肠道形态和抗氧化能力的影响,实验选取初始体质量为（7.71±0.02）g的大黄鱼幼鱼为研究对象,设置2个对照组,即高鱼粉对照组FM（鱼粉含量为45.0%）和低鱼粉对照组N0（鱼粉含量为31.5%）,在低鱼粉对照组基础上分别添加0、75、150、300、600和1 000 mg/kg的核苷酸,共配制出7种等氮等脂饲料,在海水浮式网箱中进行为期63 d的养殖实验。结果表明：饲料中添加核苷酸对大黄鱼的存活率、饲料效率、摄食率、鱼体组成和形体指标没有显著影响（P〉0.05）;但增重率随着核苷酸添加量的增加呈先升高后下降趋势,在添加量为300~600 mg/kg时显著高于其他处理组（P〈0.05）。随着核苷酸添加量的增加,血清中超氧化物歧化酶（SOD）和总抗氧化力（T-AOC）的活力呈先升高后下降的趋势,并在300 mg/kg时显著高于低鱼粉对照组（P〈0.05）。核苷酸对大黄鱼肠道肌肉层厚度和微绒毛高度有显著影响（P〈0.05）,分别在添加量为150和300mg/kg时出现最大值;而对肠道褶皱高度无显著影响（P〉0.05）。结果表明,饲料中添加核苷酸能促进大黄鱼生长,改善肠道形态结构和增强机体的抗氧化能力。以增重率为指标,利用折线模型计算得到大黄鱼幼鱼饲料中核苷酸的适宜添加量为194.91 mg/kg。
The present study was conducted to analyze the effects of dietary nucleotides on growth performance,intestinal morphology and anti-oxidative capacities of juvenile large yellow croaker（ Larimichthys crocea） [initial weight：（ 7.71 ±0.02） g]. Two control diets were set,which were high fish meal（ FM,45. 0%） supplemented diet and low fish meal（ N0,31. 5%） supplemented diet,respectively. Based on the latter,five graded levels of nucleotides were added. They were 75,150,300,600 and 1 000 mg /kg,respectively. After the 9-week growth trial,results showed that survival rate,feed efficiency,feed intake and body composition of the animals were not significantly influenced by dietary nucleotides levels（ P 〉 0. 05）.Weight gain rates（ WGR） of fish fed the diets with 300 or 600 mg /kg of nucleotides were significantly higher than those in the other groups（ P 〈 0. 05）. Dietary nucleotides significantly affected the total antioxidative capacity（ T-AOC） and superoxide dismutase（ SOD） activity in serum（ P 〈 0. 05）. Values of T-AOC and SOD increased first,and then declined as the dietary nucleotides levels increased. The highest values of these two parameters were found in the treatment with 300 mg /kg of dietary nucleotides. Dietary nucleotide significantly increased microvillus height（ HMV） and the thickness of the muscular layer（ TML） in the proximal intestine（ P 〈 0. 05）. The HMV in intestine of fish fed diet with 300 mg /kg of nucleotides was higher than that in the control group. The TML in intestine of fish fed diet with 150 mg /kg of nucleotides was higher than that in the control group. There was no significant difference in fold height（ HF） of intestines among all groups（ P 〉 0. 05）. In conclusion,dietary nucleotides can improve the growth of the large yellow croaker. Meanwhile,it can also increase the anti-oxidative capacity and improve the intestinal morphology.Based on the data of WGR,the broken-line analysis showed that the optimal level of dietar
出处
《水产学报》
CAS
CSCD
北大核心
2014年第8期1140-1148,共9页
Journal of Fisheries of China
基金
公益性行业(农业)科研专项(201003020,200903029)
关键词
大黄鱼
核苷酸
生长
抗氧化
肠道形态
Larimichthys crocea
nucleotides
growth
anti-oxidative capacity
intestinal morphology
