摘要
目的检测BMPs/BMPR/Smads信号转导通路上的BMPRⅠa人脑胶质瘤组织中的表达,研究其与人脑胶质瘤发生、发展中的作用及其与临床病理分级的关系。方法分别应用RT-PCR技术和免疫组织化学SABC法检测20例正常脑组织和40例不同级别人脑胶质瘤组织中BMPRⅠa mRNA和蛋白质表达量,并分析其与患者的年龄、性别、病理分级的相关性。结果人脑胶质瘤组织BMPRⅠa mRNA和蛋白质表达量比正常脑组织显著降低,并以Ⅲ、Ⅳ期肿瘤组织中的表达显著低于Ⅰ、Ⅱ期;上述指标的变化与患者的年龄及性别无关。结论在人脑胶质瘤发生、发展过程中BMPRⅠa可能起到抑制作用,且与年龄和性别无关。
Objective To detect the expressions of BMPRⅠ a of BMPs/BMPR/Smads signaling transduction pathway in human brain glioma,to research the role of its in tumorigenesis and progression of brain glioma and its correlation to clinical pathology.Methods The mRNA and protein expressions of BMPR Ⅰa was detected in 20 normal brain tissues and 40 samples with human glioma by RT-PCR and SABC immunohistochemical methods,repectively,and analyzed the correlation with the patient′s age,gender and pathological grade.Results Compared with normal brain tissues,BMPR Ⅰa mRNA and protein expressions in human glioma were reduced significantly,especially inⅢ andⅣ stage tumor tissues.The difference was not related with the patient′s age and gender.Conclusion In progression of glioma,BMPR Ⅰa may play a role in restraining,and has nothing to do with age and sex.
出处
《重庆医学》
CAS
CSCD
北大核心
2014年第23期3005-3007,3010,共4页
Chongqing medicine
基金
河南省科技创新杰出人才计划项目资助(084200410011)
