摘要
目的:研究DNA条形码技术在溪黄草药材品种鉴定中的适用性。方法:选取rbcL、psbA-trnH、matK和ITS2等4个条形码标记,对41份溪黄草药材样品(含溪黄草Rabdosia serra、线纹香茶菜R.lophanthoides和纤花香茶菜R.lophanthoides var.graciliflora)进行DNA提取、PCR扩增及双向测序,对测序结果进行质量检查、人工校对和序列拼接获取标准碱基序列信息,通过比较序列获得成功率以及物种鉴定力(TaxonGap法),筛选适合溪黄草基原鉴定的DNA条形码序列。结果:条形码序列获得成功率依次为rbcL(100%)、psbA-trnH(90.2%)、ITS2(87.8%)和matK(70.7%);物种鉴定力方面,rbcL、psbA-trnH和ITS2等3条序列均可有效鉴定不同物种,但不适于分辨种下单位。结论:综合考察序列易得性和物种鉴定力,rbcL可作为溪黄草品种鉴定的首选条形码。
This study was aimed to identify original plants of Xi-Huang-Cao （XHC） through DNA barcodes. The nucleotide sequence information of rbcL, psbA-trnH, matK and ITS2 regions were abstracted using standardized manners from 41 samples （including Rabdosia serra, R. lophanthoides and R. lophanthoides var. graciliflora）. Sequencing efficiency of each marker was calculated. Species identification capability was tested on the basis of TaxonGap. The results showed that sequence success rates were 100% for rbcL, 90.2% for psbA-trnH, ＇87.8% for ITS2, and 70.7% for matK. Three markers （rbcL, psbA-trnH and ITS2） were competent for species discrimination （not for subspecies）. It was concluded that rbcL can be the preferred barcode for XHC because of its convenience and efficacy.
出处
《世界科学技术-中医药现代化》
北大核心
2014年第7期1487-1490,共4页
Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology
基金
广东省科技厅科技计划项目(2012A061600005):广东省岭南药用植物资源保护与利用企业重点实验室(产学研)培育基地
负责人:王德勤
国家自然科学基金委青年基金项目(81102764):基于改进Mauve算法的贯众类药材DNA条形码序列预测与验证
负责人:马新业
广东省科技厅博士启动项目(S2011040005237):广东省水龙骨科药用植物资源DNA条形码鉴定研究
负责人:马新业
广东省教育厅高等院校学科与专业建设专项(重点实验室滚动支持)(2013CXZDA011):春砂仁等"十大广药"DNA条形码标准化研究
负责人:陈蔚文
