摘要
十二烷基肌氨酸钠与天然细胞色素c通过自组装方式构建了一种新型纳米胶团结构人工过氧化物酶(Artificial Peroxidase,AP)。采用紫外可见光分光光度计对AP酶促反应过程进行研究。表明AP在较宽的pH范围具有活性,并在pH 5.0,100 mmol/L磷酸盐缓冲液中达到最大。该AP的米氏常数Km、催化速率kcat以及催化效率分别为1.70μmol/L、0.11 s-1、0.065μM-1s-1,催化效率为天然辣根过氧化物酶的90.2%。
A nanostructured artificial peroxidase （ AP ） was constructed self -assembly using cytochrome c and sodium N-lauroyl sarcosine nano miceller.UV-vis spectroscopic study showed that the AP had peroxidase activity in a wide pH range with maximum peroxidase activity at pH 5.0.The Michaelis -Menten （Km）, catalytic rate （kcat） and catalytic efficiency were obtained to be 1.70 μmol/L, 0.11 s-1 , 0.065 μM-1 s-1 , respectively.The catalytic efficiency of the AP was 90.2%as efficient as the native horseradish peroxidase （ HRP）.
出处
《广州化工》
CAS
2014年第16期34-37,共4页
GuangZhou Chemical Industry
基金
河南省科技发展计划项目(No.142300410124)
河南大学特聘教授科研启动资金(No.5443D08010)
伊朗国家科学基金(INSF)
