摘要
目的检测缺血性脑卒中患者血清中视锥蛋白样蛋白1(Visinin-like protein-1,VILIP-1)水平并探讨其早期临床诊断价值。方法用酶联免疫吸附试验(ELISA)检测VILIP-1水平并进行诊断性能分析,研究对象为可疑短暂性脑缺血发作(TIA)(出现症状24 h内)或急性缺血性脑卒中(出现症状72 h内)患者340例、健康体检者102例和具有血管危险因素的对照者98例。结果急性缺血性脑卒中患者(IS)组VILIP-1为9.8(1.9～14.2)μg/L,显著高于所有对照组(P<0.05)。VILIP-1在发病最初3 h内显著升高。受试者工作特征曲线(Receiver Operating Characteristic,ROC)显示当诊断界值为0.5μg/L时,VILIP-1区别IS和其他对照者的敏感性和特异性分别为90.5%和71.5%,IS组与单独对照组鉴别时也具有较高的敏感性和特异性,诊断效能较强。结论 VILIP-1是早期诊断IS比较敏感和特异性的指标,方法易行,临床价值较高。
Objective To investigate the concentration of VILIP-1 in serum of patients with ischemic stroke,and to explore its clinical value in early diagnosis of ischemic stroke. Methods Three hundred and forty suspicious transient ischemic attack patients（TIA） or acute ischemic stroke patients（IS）,102 healthy people and 98 controls with vascular risk factors were selected to detect the concentrations of serum VILIP-1 by enzyme linked immunosorbent assay（ELISA）,and the diagnostic performance of VILIP-1 in these cases was analyzed. Results The serum level of VILIP-1 in IS patients was 9. 8（1. 9 ～14. 2） μg/L,higher than other three groups（P 0. 05）. Serum VILIP-1 level significantly increased in IS patients within3 h of symptom onset. Receiver operating characteristic curve（ROC） showed that when the diagnosis critical value of VILIP-1was 0. 5 μg/L,it could differentiate ischemic stroke from other three control groups with the sensitivity of 90. 5% and specificity of 71. 5%,indicating its high diagnostic efficacy. It was also found that VILIP-1 could also differentiate IS from either the risk factors group or healthy control group,with high sensitivity and specificity. Conclusion VILIP-1 was a sensitive and specific biomarker for early diagnosis of IS,and easy to operate,with high clinical value.
出处
《中国卫生检验杂志》
北大核心
2014年第15期2135-2137,共3页
Chinese Journal of Health Laboratory Technology
基金
河南省医学科技攻关计划基金资助项目(201303135)
关键词
视锥蛋白样蛋白1
血清学标志物
缺血性脑卒中
Visinin - like protein - f（ VILIP - 1 ）
Serological markers
Ischemic stroke
