摘要
黄檗种子具休眠特性,且休眠程度因种子产地和年份不同有较大差异,常规低温层积解除种子休眠的方法在实际生产中效果不甚理想。为了解决黄檗种子的萌发率低的问题,探究最有效的打破种子休眠方式,采用了各种化学引发剂浸种处理,以及协同4℃或8℃低温层积处理。结果表明,NAA、6-BA、KNO3、PEG6000溶液浸种可部分解除种子的休眠,并显著提高种子发芽率;用引发剂溶液浸种后4℃层积30天可有效解除种子休眠,其中GA3(1000 mg/L)、NAA(80 mg/L)、6-BA(400 mg/L)、KNO3(1.0%)、PEG6000(20 g/L)溶液浸种后4℃层积30天可有效解除种子休眠,分别将黄檗种子发芽率分别从11.50%升高到91.00%、94.00%、97.00%、94.50%、83.00%。引发剂可有效打破黄檗种子休眠,促进种子萌发并能缩短低温层积时间。
Seed of Phellodendron amurense Rupr presented different degrees of dormancy which varied with provenance and year. Conventional cold stratification was only partially effective in breaking dormancy of treated seeds. In order to solve the problem of low rate of seed germination of Phellodendron amurense Rupr, explore the most effective way to break seed dormancy, different chemical-initiators were used to treat the seeds, and then combined with 4℃ or 8℃ cold stratification. In this paper, the results showed that soaked with 6-BA, KNO3, PEG 6000 could promote seed germination. In addition, pretreated with 1000 mg/L GA3, 80 mg/L NAA, 400 mg/L 6-BA, 1.0% KNO3, 20 g/L PEG6000 that combined with 4℃ cold stratification for 30 d could break seed dormancy more effectively and make the germination rate increased from 13.50% to 91.00%, 94.00%, 97.00%, 94.50%, and 83.00% respectively. The priming could effectively break the seed dormancy and promote seed germination of Phellodendron amurense Rupr and shorten the time of cold stratification.
出处
《中国农学通报》
CSCD
2014年第22期209-216,共8页
Chinese Agricultural Science Bulletin
基金
国家"重大新药创制"科技重大专项(2009ZX09308-002)
"中药材种子种苗和种植(养殖)标准平台"(2012ZX09304006)
关键词
黄檗
种子
休眠解除
低温层积
引发剂
关黄柏
PheUodendron amurense Rupr.
seed
dormancy breaking
cold stratification
priming
Phellodendri amurensis Cortex
