摘要
目的:观察艾叶水提物及其发酵物对白色念珠菌性阴道炎的治疗作用。方法:采用小鼠阴道内接种1×107cfu/mL白色念珠菌悬液20μL,建立白色念珠菌阴道炎模型。将成模小鼠随机分为模型对照组、艾叶水提物组[(生药,下同)20g·L-1]、艾叶发酵物低、中、高剂量组[(生药,下同)10,20,40 g·L-1]及氟康唑组(2 g·L-1),另设空白对照组,每组15只。镜检各组小鼠阴道灌洗液白色念珠菌及脱落上皮细胞,并计算其转阴率,测定阴道灌洗液菌落形成单位(CFU),HE染色观察各组小鼠阴道组织病理改变。结果:模型对照组各小鼠阴道灌洗液均可见白色念珠菌感染,但空白对照组未见。与模型对照组比较,给药后第5,8天艾叶发酵物各剂量组及氟康唑组均能显著增加白色念珠菌孢子及脱落细胞转阴率,降低CFU数量,减轻阴道组织损伤(P<0.05,P<0.01),且艾叶发酵物于给药第8天,中、高剂量组上述指标结果更显著(P<0.01);而艾叶水提物组与模型对照组比较上述各指标无差异。结论:艾叶发酵物对白色念珠菌阴道炎小鼠具有显著治疗作用,艾叶水提物疗效较艾叶发酵物差。
Objective：To observe effects of Artemisiae Argyi Folium aqueous extract and its ferment substance on candida albicans vaginitis in mice.Method：The Candida albicans vaginitis model was induced by the vagina in mice which were vaccinated 1 × 107cfu/mL C.albicans suspension 20 μL.The model mice were randomly divided into control group,model group,Artemisiae Argyi Folium aqueous extract group （20 g ·L-1）,Artemisiae Argyi Folium ferment substance （AAFS） groups with different doses （10,20,40 g · L-1） and fluconazole group （2 g ·L-1）,15 mice each group.The mice in each group were given therapeutic drugs by vagina vaccination for 7 d,once per day.The candida albicans and epithelial cells in vaginal lavage liquid were determined by microscopic examination.HE staining observed pathological changes of vaginal tissue.Result：The candida albicans infection was found in model group,but not in blank control group.Compared with the model group,AAFS with different doses and fluconazole could remarkably increase negative-converting rate of candida albicans and epithelial cells,decrease CFU number,and reduce the damage of vaginal tissue at 5 or 8 day after the treatment （P ＜0.05,P ＜0.01）,and AAFS with middle and high doses had more remarkable effects at 8 d after administration （P ＜ 0.01）,but Artemisia Argyi aqueous extract group had no difference on above indexes.Conclusion：Compared with Artemisia Argyi aqueous extract,AAFS had significant therapeutic effects on C.albicans vaginitis in mice.
出处
《中国实验方剂学杂志》
CAS
北大核心
2014年第16期131-134,共4页
Chinese Journal of Experimental Traditional Medical Formulae
基金
唐山市科技计划项目(12130208A-2)
关键词
艾叶
发酵
白色念珠菌
阴道炎
Artemisiae Argyi Folium
ferment
Candida albicans
vaginitis
