摘要
目的：建立榆钱中水杨酸含量的测定方法.方法：采用甲醇提取,HPLC测定,Hypersil C18色谱柱（4.5 mm× 200mm,5μm）,流动相为甲醇-0.3％磷酸溶液（36∶64）,流速1.0 mL· min-1,检测波长为327 nm,柱温30℃,进样量为20 μL.结果：该方法样品中水杨酸与干扰物质分离良好,水杨酸质量浓度在8.82～282.0 mg·L-1与峰面积呈良好的线性关系（r=0.999 8）,供试品溶液在8h内稳定,回收率为95.23％-105.3％,RSD分别为1.50％,4.85％,4.68％.结论：该方法简便、可靠、重复性好,可用于测定榆钱中水杨酸的含量.
Objective：To develop a novel method for the determination of salicylic acid in fruits of elm.Method：The HPLC separation was performed on an Elite Hypersil C18column （4.5 mm × 200 mm,5 μm） at a flow rate of 1.0 mL ·min-1 and column temperature of 30 ℃.Detection was monitored at 327 nm.The mobile phase was methanol-0.3％ phosphoric acid （36∶64）.Result：Salicylic acid showed good linearity from the concentration range of 8.82 to 282.0 mg ·L-1 （r =0.999 8）.The sample solution is steady within 8 h.The recovery was 95.23％-105.3％,RSD was 1.50％,4.85％,4.68％.Conclusion：The method is simple,reliable and reproducible.It can be used in the determination of salicylic acid in fruits of elm.
出处
《中国实验方剂学杂志》
CAS
北大核心
2014年第16期112-114,共3页
Chinese Journal of Experimental Traditional Medical Formulae
基金
河北省自然科学基金项目(C2013405094)
