摘要
目的:优选金樱子抗糖尿病活性部位的提取工艺。方法:以体外抗α-淀粉酶活性和抗氧化活性的综合评分为因变量,乙醇体积分数、料液比、提取时间、提取次数为自变量,采用Design Expert 8.05软件分析试验数据,建立多元二次回归方程,采用Box-Behnken响应面法优选金樱子抗糖尿病活性部位的提取工艺。结果:最佳提取工艺为加11倍量85%乙醇提取2次,每次120 min;α-淀粉酶活性平均抑制率95.39%,RSD 4.59%,DPPH自由基平均清除率91.47%,RSD 4.12%,综合评分0.967 2,与预测值0.977 8吻合程度良好。结论:通过生物活性评价优选的提取工艺更科学合理,符合临床实际应用。
Objective：To optimize extraction process of anti-diabetic active parts from Rosae Laevigatae Fructus.Method：With composite score of in vitro anti-α-amylase and anti-oxidation activities as dependent variable,ethanol concentration,solid-liquid ratio,extraction time and times as independent variables,test data were analyzed by Design Expert 8.05 software,then multivariate quadratic regression equation was established,Box-Behnken response surface methodology was adopted to optimize extraction process of anti-diabetic active parts from Rosae Laevigatae Fructus.Result：Optimal process parameters were as follows：extracted twice with 11 times the amount of 85％ ethanol for 120 min per time; average inhibition rate of α-amylase was 95.39％ with RSD of 4.59％,average clearance rate of DPPH was 91.47％ with RSD of 4.12％,composite score was 0.967 2,which was fit with its predictive value of 0.977 8.Conclusion：Optimization of extraction process was scientific and conformed with clinical practical application by biological activity evaluation.
出处
《中国实验方剂学杂志》
CAS
北大核心
2014年第16期26-29,共4页
Chinese Journal of Experimental Traditional Medical Formulae
基金
国家自然科学基金项目(81374028
30902002
81273750)
陕西省中医药管理局项目(13-ZY014)
陕西省重点科技创新团队计划项目(2013KCT-26)
