摘要
[目的]本研究测定了摩拉水牛、尼里水牛和槟榔江水牛的射精量、精子密度、精子活力和畸形率。[方法]精液品质按照《牛冷冻精液》标准和《牛冷冻精液生产技术规程》进行测定。[结果]结果得出,摩拉水牛公牛的射精量为5.1±2.37ml(n=2688)、精子密度为7.8±3.68亿/ml、原精活力57.4±16.42%;冷冻精液解冻活力平均为36.0±2.13%(n=1680)、畸形率为20.62±11.04%(n=873);尼里水牛公牛的射精量为5.3±1.99ml(n=1370)、精子密度为8.4±3.54亿/ml、原精活力62.7±12.51%;冷冻精液解冻活力平均36.5±2.43%(n=1102)、畸形率为15.2±5.71%(n=481);槟榔江水牛公牛的射精量为5.6±2.26ml(n=271)、精子密度为8.6±5.13亿/ml、原精活力65.2±9.19%;冷冻精液解冻活力平均为37.2±2.48%(n=239)、畸形率为19.6±6.08%(n=110)。[结论]本研究获得了水牛精液品质的基础数据。
[Objective] Ejaculate volume, sperm density, sperm motility and deformity rate of Murrah buffalo, Nili Buffalo, and Binglangjiang River buffalo were measured in this study. [Methods] The semen qualities were appraised in accordance with the ＂standard of cattle frozen semen＂ and＂ frozen bull semen production technology regulations＂. [Result] The results showed that： ejaculate volume, sperm densit and sperm motility of Murrah buffalo bulls were 5.1 ～ 2.37ml （n=2688）, 0.78 ～0. 368 billion/ml, an y d 57.4 ± 16.42%, respectively. And the mean vitality and abnormal rate of thawing of frozen semen of Murrah buffalo bulls were 36 ～2.13% （n=1680）, and 20.62 ～ 11.04% （n=873）, respectively. Ejaculate volume, sperm density and sperm motility of Nili buffalo bulls were 5.3 ～ 1.99ml （n=1370）, 0.84 ±0. 354 billion/ml, and 62.7 ±12.51%, respectively. And the mean vitality and abnormal rate of thawing of frozen semen of Nili buffalo bulls were 36.5 ～ 2.43% （n=1120）, and 15.2 ±5.71% （n=481）, resp 5.6 ectively. Ejaculate volume, sperm density and sperm motility of Binglangjiang River buffalo bulls were ±2.26ml （n=271）, 0.86 ±0. 513 billion/ml, and 65.2±9.19%, r tality and abnormal rate of thawing 48% （n buffalo of frozen semen of Binglangjiang River espectively. buffalo bulls And the mean viwere 37. 2 ～2. 239）, and 19.6 ±6.08% （n=110）, respectively. [Conclusion] In this study, the basic data of semen quality were collected.
出处
《中国牛业科学》
2014年第4期21-25,共5页
China Cattle Science
基金
云南省科技计划(2008LA018)
现代农业奶牛产业技术体系建设专项资助
关键词
水牛
采精
饲养管理
冷冻
解冻
Buffalo semen collection feeding management
freezing thawing
