摘要
为研究马立克病毒强毒感染与疫苗免疫鸡组织病理学变化的差异,试验采用108只1日龄海兰褐公雏鸡,随机分为对照组(C组)、马立克病毒CVI988疫苗株腹腔注射免疫组(IA组)和京-1株攻毒组(V组)。在接种后3、6、10、15、20、26、35、45、60d,对3组各取4只鸡进行剖检,分别取肺脏、胸腺、骨骼肌、肾脏、睾丸、肝脏、心肌、腺胃、脾脏、法氏囊、坐骨神经等组织于100mL/L中性福尔马林中固定,石蜡包埋,制备切片,HE染色,光镜下进行组织病理学观察。结果与对照组相比,V组在接种6d时,无明显病理变化,IA组淋巴器官内出现淋巴细胞增多;接种10d后,V组脾脏动脉周围淋巴鞘、淋巴小结内淋巴细胞数量减少;15d后胸腺小体消失,出现肿瘤细胞,其他组织均出现增生性团块,有大小不等的肿瘤细胞;IA组可见脾脏中淋巴细胞增多,动脉周围淋巴鞘脾小结增多,胸腺小体中淋巴细胞增多,其他组织有明显的淋巴细胞浸润。由此可见,鸡感染马立克病毒后免疫器官损伤,各组织器官都出现明显病理学变化;鸡接种疫苗后免疫器官淋巴细胞逐渐增多,但以后也出现免疫器官损伤与其他组织器官淋巴细胞增多,由此表明了CVI988疫苗的免疫毒性,为研究无免疫毒性且有较好免疫力的马立克病疫苗提供理论依据。
This study was designed to investigate the influence of Gallid herpesvirus 2Marek＇s Disease challenge virus and atteunated vaccine virus CVI988/Rispens on changes of histopathology in chickens.108 one day old chicks were randomly divided into a control group（C）,an experimental group challenged with Jing-1challenge strain（V）,and a third experimental group injected intra-abdominally（IA）with vaccine strain,CVI988/Rispens.Different organs of lung,thymus,skeletal muscle,kidney,testis,liver,cardiac muscle,stomachus glandularis,spleen,bursa of Fabricius,sciatic nerve were removed immediately from 4chicks of every group sacrificed by cervical dislocation on days 3,6,10,15,20,26,35,45 and 60,and were fixed overnight in 100mL/L neutral formalin,and embedded in paraffin wax for routine histological procedures.Paraffin sections were stained with hematoxylin and eosin.The histopathological changes were evaluated with light microscope.It was found that group V has no pathological changes in six days,group IA showed lymphocytosis.On day 10,the number of lymphocytes was declined in periarterial lymphatic sheath and lymph nodule in group V.Thymus corpuscles disappeared,other organs were hyperplasia and tumour cells in various size appeared in 15 days.Group IA showed lymphocytosis,splenic corpuscles increased in periarterial lymphoid sheath,lymphocytosis in thymus corpuscles and increased lymphocytes soaked in other tissues.The results suggest that pathological changes appeared in immune organs in chickens challenged with Marek＇s disease virus,obvious pathological changes also appeared in other tissues.While increased lymphocytes concentrated in immune organs of vaccinated chickens,but subsequently immune organs were damaged and obvious increased lymphocytes appeared in other organs.In conclusion,atteunated vaccine virus CVI988 Rispens had its immunotoxicity.So it is necessary for preparing nontoxicity vaccine in the future.
出处
《动物医学进展》
CSCD
北大核心
2014年第8期36-39,共4页
Progress In Veterinary Medicine
基金
国家自然科学基金(31072179)
山西省自然科学基金(2009011043-1)
山西省科技攻关项目(20130311027-3)
山西省高等学校大学生创新创业训练项目(2011088)
山西农业大学第九批大学生科技创新项目(9-007
9-011)
