摘要
目的检测散发性大肠癌患者癌组织及粪便脱落细胞中APC基因的突变,探索粪便基因检测代替癌组织检测的可行性。方法运用蛋白截断技术(PTT),对50例散发性大肠癌患者粪便脱落细胞及癌组织中APC基因第15外显子进行突变检测。结果大肠癌患者的癌组织中APC基因突变检出17例,突变率为34%(17/50);患者粪便脱落细胞APC基因突变检出14例,突变率为32.6%(14/43);癌组织及粪便脱落细胞APC基因检测突变率相比差异无统计学意义(P>0.05)。Kappa值为0.749,大肠癌患者的癌组织中APC基因及粪便脱落细胞APC基因检测的一致性较好。结论大肠癌患者粪便脱落细胞中APC基因突变与癌组织APC基因突变一致性较好,粪便脱落细胞APC基因检测有望成为大肠癌早期诊断的方法之一。
Objective To detect APC gene mutations in sporadic colorectal cancer tissue and exfoliated cells in stool,and to explore the feasibility of stool gene detection instead of tissues.Methods The protein truncation test was used to analyze the fifteenth exon mutations of APC gene of cancer tissues and exfoliated cells in stools of fifty cases with sporadic colorectol cancer.Rusults APC gene mutations were found in tumor tissues of 17 eases and the mutation rate was 34% （17/50） while APC gene mutations were found in exfoliated cells in stool of 14 cases and the mutation rate was 28% （14/43）.There was no difference between the mutation rate of tumor tissue and exfoliated cells in stool （P 〉 O.05）.Kappa value was 0.749.Conclusion The mutation of APC gene in tumor tissues and exfoliative cells in stool are preferably agreement.The detection of APC gene mutation in exfoliated cells in stool can be used as one of a screening method for early diagnosis of colorectal cancer.
出处
《宁夏医科大学学报》
2014年第4期364-367,共4页
Journal of Ningxia Medical University
基金
国家自然科学基金(30960370)
宁夏科技攻关项目(2009)
