摘要
目的评价滇龙胆不同居群花的药材质量,探讨滇龙胆花是否可以与其根同等入药。方法采用高效液相色谱法测定滇龙胆花中活性成分龙胆苦苷、獐牙菜苦苷、獐牙菜苷含量,建立其高效液相色谱法(HPLC)指纹图谱及其与根比较的HPLC指纹图谱,并用单因素方差分析、聚类分析和主成分分析对其品质及质量等同性进行评价。结果大理白族自治州滇龙胆不同居群花间整体品质较好,龙胆苦苷含量较低的感通居群龙胆苦苷含量也与国家标准接近,滇龙胆不同居群间花中龙胆苦苷、獐芽菜苦苷、獐芽菜苷含量具有极显著差异(P<0.01);对不同居群花间质量等同性进行分析,确定HPLC指纹图谱中15个共有峰,滇龙胆不同居群花间HPLC指纹图谱相似度均在0.809以上,指认其中龙胆苦苷、獐牙菜苦苷、獐牙菜苷3个与活性相关的特征峰,不同居群花间质量等同性基本一致;对不同居群间花与根进行指纹图谱分析,确定HPLC指纹图谱中8个共有峰,共有峰HPLC指纹图谱相似度除了感通居群外,均在0.846以上,说明花中所含有效成分与根相似,并且还有些根中未检测到的成分;滇龙胆不同居群花间龙胆苦苷、獐牙菜苦苷、獐牙菜苷总含量聚类分析和HPLC指纹图谱主成分分析结果相似。结论从滇龙胆花中活性成分含量已达到药典标准要求。但是滇龙胆花与根比较的HPLC指纹图谱存在一定的差异。因此滇龙胆花是否可以作为其根的替代品入药,仍需进一步做药理、毒理及临床实验。
OBJECTIVE To evaluate the quality of the flowers of Gentiana rigescens Franch. from different populations in order to explore whether the flowers can be used as a medicine as the roots. METHODS The contents of the active ingredients, ie, gentiopicro- side, swertiamarin and swerosidc, in the flowers of Gentiana rigescens Franch. were determined by HPLC. The HPLC fingerprint of the flowers were established and compared with that of the roots. The quality and quality equivalence were evaluated by One-way ANOVA, cluster analysis and principal component analysis （PCA）. RESULTS The overall quality of the flowers of C.entiana rigescens Franch. from different populations in Dali Yunnan was good. The content of gentiopicroside in the sample from Gantong population was lower than those in the other populations, while it was close to the national standard. There was significant difference in the contents of gentiopicro- side, swertiamarin, and sweroside in the flowers of Gentiana rigescens Franeh from different populations （ P 〈 0. 01 ）. Quality equivalence of Gentiana rigescens Franeh. from different populations was analyzed, and 15 common peaks in HPLC fingerprint were selected to evalu- ate the similarities of the crude drugs. The similarities of the common peaks were all above 0. 809. Three characteristic peaks of gentiopi- croside, swertiamarin and sweroside which were related to the activity of Gentiana rigescens Franch. were identified, and the quality of different populations was basically equivalent. The HPLC fingerprints of the flowers of Gentiana rigescens Franch. from different popula- tions were compared with that of the roots. And 8 common peaks were selected to evaluate the similarities of the crude drugs. The similar- ity of the common peaks was all above 0. 846 except to Gantong population. The total contents of gentiopicroside, swertiamarin and swero- side of C, entiana rigescens Franch. from different populations were similar as shown by cluster analysis and PCA of HPLC fingerprint. CONCLUSION T
出处
《中国药学杂志》
CAS
CSCD
北大核心
2014年第16期1451-1457,共7页
Chinese Pharmaceutical Journal
基金
云南省教育厅基金重点项目(2013Z153)
大理学院2012年应用开发基金资助项目
大理学院2011年中青年学术带头人培养项目
关键词
滇龙胆花
高效液相色谱法
含量测定
指纹图谱
质量标准
品质评价
Gentiana rigescens Franch. flowers
HPLC
content determination
fingerprint
quality standard
quality evaluation
