摘要
目的:以肌注氢化可的松建立肾阳虚大鼠模型为研究平台,通过血管紧张素Ⅱ(AngⅡ)、血管紧张素受体1(AT1R)指标探讨温补肾阳法对肾阳虚模型大鼠体液调节系统的影响,也为肾主水理论寻找科学依据。方法:60只健康雄性SD大鼠,随机分为对照组、模型组、干预组,对照组不造模,给予生理盐水灌胃[10ml/(kg·d)];模型组注射氢化可的松[2ml/(kg·d)],造模后给予右归胶囊混悬液灌胃[10ml/(kg·d)],干预组造模后给予生理盐水灌胃[10ml/(kg·d)]。第14天三组各处死10只,第42天,各组再处死剩余的大鼠,取肺脏组织常规切片,免疫组化法检测AngⅡ、AT1R在肺脏的表达。结果:免疫组化法检测AngⅡ、AT1R蛋白阳性反应产物呈现棕黄色,第14天,与对照组比较,模型组和干预组大鼠肺组织AngⅡ表达减少,AT1R表达也减少,差异有统计学意义(P<0.05);第42天时,与模型组比较,干预组大鼠肺组织AngⅡ和AT1R表达较多,差异有统计学意义(P<0.05)。结论:肾阳虚大鼠肺组织AngⅡ、AT1R的表达明显下降,表明肾阳虚大鼠体内体液代谢血液循环受到影响;温补肾阳名方右归胶囊可以通过增加模型大鼠肺组织AngⅡ和AT1R的表达,从而缓解肾阳虚对模型大鼠体液调节系统紊乱的影响。
Objective： To study the effect of prescription for warming and recuperating the kidney-yang on angiotensin receptor （Ang Ⅱ ） and angiotensin receptor 1 （AT1R） in hydrocortisone induced rat model of kidney yang deficiency. Methods： Sixty healthy male SD rats were randomly divided into control group, model group, and Yougui capsule intervention group. Immunohistochemistry was used to detect the expression of Ang Ⅱ and ATIR in lung tissue. Results： Both Ang Ⅱ and AT1R expression levels in lung were lower in model group and intervention group of than in control group after 14 days （P〈0.05）. But after 42 days＇ treatment, both Ang Ⅱ and ATIR ex- pression levels were higher in Yougui-intervention group than in model group （P 〈 0. 05）. Conclusion： The prescription for warming and recuperating the kidney-yang could regulate the dysfunction of kidney yang deficiency rats through up-regulating the expression of Ang Ⅱ and AT1R.
出处
《武汉大学学报（医学版）》
CAS
北大核心
2014年第4期538-540,共3页
Medical Journal of Wuhan University
基金
国家自然科学基金资助项目(编号:81202615)
关键词
温补肾阳法
肾阳虚
AngⅡ
AT1R
Prescription for Warming and Recuperating the Kidney-Yang
Kidney-Yang Deficiency
Angiotensin Ⅱ
Angiotensin Receptor 1
