摘要
为研究甲基化CpG结合域蛋白2(methyl-CpG binding domain protein 2,MBD2)在围植入期小鼠子宫内膜的表达规律,通过采用实时荧光定量PCR(Real-time fluorescence quantitative PCR,qPCR)、Western blot和免疫组化技术检测未孕小鼠(d0)和不同孕天小鼠子宫MBD2的表达情况。qPCR结果显示,d0至d7的小鼠子宫内膜组织均有MBD2 mRNA表达,在d5至d7高表达。MBD2蛋白在子宫内膜的表达规律与qPCR结果相符。MBD2蛋白在孕d1到d4中度表达于腔上皮、腺上皮和基质细胞,在d5至d7基质细胞表达增强,主要表达于蜕膜区。假孕小鼠子宫内膜中,MBD2在腔上皮、腺上皮和基质细胞中中度表达,d5至d7基质细胞表达明显减弱。动物模型中,宫角注射MBD2基因反义寡聚脱氧核苷酸,可抑制MBD2的表达,降低人工诱导蜕膜化反应和蜕膜化标志物PRL的表达。MBD2在早孕小鼠子宫内膜的表达模式提示其可能参与了蜕膜化过程。
The experiment was aim to explore the expression pattem of Methyl-CpG binding domain protein 2 （MBD2） during peri-implantation in the mouse endometrium. Expression of MBD2 mRNA and MBD2 protein in the non-pregnant and pregnant mouse endometrium from dO to d7 was examined by Real-time fluorescence quantitative PCR （qPCR）, Western blot and immunohistochemistry. MBD2 mR.NA expressed in uterine endometrium of the non-pregnant （dO） and pregnant mouse （dl to d7）. MBD2 mRNA increased gradually from d5 to d7. Western blot analysis showed that the protein expression of MBD2 in mouse endometrium had the similar expression pattern to its mRNA that detected by Real-time fluorescence quantitative PCR. Moreover, from pregnant dl to d4, MBD2 was expressed in epithelium, glandular cells and stromal cells in the endometrium. On d5 to d7, MBD2 was more intensely expressed in stromal cells, especially in the decidual zone. The expression of MBD2 in the pseudopregnant on dl and d4 was expressed in epithelium, glandular cells and stromal cells in the endometrium. But it was significantly lower in the stromal on d5 to d7. Using artificial decidualization and treatment withantisense oli-godexynucleotides of MBD2, the expression of MBD2 in the uterus was remarkably inhibited and the level of decidualization was decreased. These results suggested that MBD2 was involved in modulating the decidulization of mouse uterine.
出处
《中国细胞生物学学报》
CAS
CSCD
北大核心
2014年第7期976-982,共7页
Chinese Journal of Cell Biology
基金
国家自然科学基金(批准号:81370731)资助的课题~~
