摘要
以甘油为SBR反硝化除磷的碳源,研究了甘油作为反硝化除磷碳源的可行性,以及不同初始pH与进水COD/P对反硝化除磷效果的影响。并采用高通量测序技术对反应器中驯化期与稳定期的菌群结构变化进行分析。结果表明,甘油作为反硝化除磷的碳源具有可行性,除磷效率达到79.2%,平均出水TP为0.98 mg/L。pH为7.6、COD/P=20左右时处理效果较好。以甘油为碳源驯化的除磷污泥中,在"目"级别上的主导菌群以红环菌目(Rhodocyclales)为主,从驯化到稳定,其比例由24.8%增加至42.4%。通过与已知除磷菌序列比对(BLAST),在序列相似度为97%条件下,种泥中除磷菌序列比例为0.71%,随着污泥驯化,除磷菌序列比例由驯化期的1.6%提高至稳定期的8.0%。
Feasibility of glycerol as the carbon source for denitrifying phosphorus removal system was studied, and effects of initial pH as well as influent COD/P were investigated. Succession of microbial community structure was studied by high throughput sequencing. Results showed that glycerol was a feasible carbon source for denitdfying phosphorus removal, with average effluent TP of 0.98 mg/L and phosphorus removal rate of 79.2%. The optimal pH was 7.6 and COD/P was about 20. Rhodocyclales was dominated in all the samples on the order level, with its percentage increased obviously from 24.8% at acclimation period to 42.4% at stable period. Through sequence BLAST with the known sequence of phosphorus removal bacteria, 0.71% of sequence of seed sludge could be assigned to phosphorus removing bacteria at similarity of 97%, and for the glycerol SBR sludge, the proportion of phosphorus removal bacteria sequences was increased from 1.6% at acclimation period to 8.0% at stable period.
出处
《环境科学与技术》
CAS
CSCD
北大核心
2014年第8期118-122,共5页
Environmental Science & Technology
基金
国家水体污染与控制重大水专项(2012ZX07313001)
深圳市基础研究项目(JC201105160582A)
关键词
反硝化除磷
甘油
多样性
菌群结构
denitrifying phosphorus removal
glycerol
diversity
community structure
