摘要
目的:监测新生儿缺氧缺血性脑病(HIE)血清钙、NO、NSE、S100B的水平变化,进一步采用神经节苷脂钠干预,证实神经节苷脂钠在新生儿HIE治疗中临床应用价值及前景。方法:设对照组为非HIE患儿(无窒息、无重度感染),并依次设常规治疗组和神经节苷脂钠治疗组,采用偶氮砷终点法、硝酸还原酶法及化学放光免疫法对生后7天及不同程度HIE患儿进行血清钙、NO、NSE、S100B水平检测。结果:新生儿HIE组血清钙较对照组降低,NSE和S100B明显高于对照组,差异有统计学意义(P<0.01);与轻度组相比,中、重度HIE组血清钙含量降低,血清NO、NSE和S100B含量增高,且组间差异有统计学意义(P<0.01);经神经节苷脂钠治疗组与常规治疗组相比,神经节苷脂钠治疗后,HIE新生儿血清钙、NO、NSE和S100B较常规治疗组浓度变化明显,差异有统计学意义(P<0.01)。结论:血清NO、NSE和S100B水平在HIE发病机制中起一定作用,其水平的增高与该病的严重程度相关,可作为早期辅助诊断HIE及判定病情严重程度的可靠指标;神经节苷脂钠可降低HIE所致的脑细胞损伤,具有重要的临床价值及应用前景。
Objective： To monitor the changes of serum calcium, nitric oxide （ NO）, neuron - specific enolase （NSE） and S100B levels in neonates with hypoxic -ischemic eneephalopathy （HIE）, further adopt ganglioside sodium intervention to confirm the clinical application value and prospect of ganglioside sodium in treatment of neonatal HIE. Methods ： The neonates without asphyxia and severe infection were selected as control group; the neonates with HIE treated with routine therapy and ganglioside sodium respectively were selected as rou- tine therapy group and ganglioside sodium group; arsenazo endpoint method, nitrate reductase method and chemiluminescence immunoassay were used to detect serum calcium, NO, NSE and S100B levels in HIE neonates of different degrees on the seventh day after birth. Results： Serum calcium level in neonatal HIE group was statistically significantly lower than that in control group, while serum NSE and S100B levels in neonatal HIE group were statistically significantly higher than those in control group （P 〈 0. 01 ） ; compared with mild HIE group, serum calcium levels in moderate HIE group and severe HIE group decreased, serum NO, NSE and SI00B levels in moderate HIE group and severe HIE group increased, there were statistically significant differences （P 〈 0. 01 ） ; compared with routine therapy group, the changes of serum calcium, NO, NSE and S100B levels in ganglioside sodium group were more obvious, there were statistically significant differences （P 〈 0. 01 ） . Conclusion： Serum NO, NSE and S100B levels play certain roles in pathogenesis of HIE, the increases of NO, NSE and S100B levels are correlated with severity of the disease, which can be used as reliable indexes for early auxiliary diagnosis of HIE and predicting severity of the disease; ganglioside sodium can reduce brain cells injury induced by HIE, which has important clinical value and application prospect.
出处
《中国妇幼保健》
CAS
北大核心
2014年第23期3769-3771,共3页
Maternal and Child Health Care of China
关键词
缺氧缺血性脑病
神经节苷脂钠
血清钙
NO
NSE
S100B
Hypoxic - ischemic encephalopathy
Ganglioside sodium
Serum calcium
Nitric oxide
Neuron - specific enolase
S100B
