摘要
目的观察中药治疗对IgA肾病(IgAN)患者T淋巴细胞亚群的影响。方法选取2011年6月—2013年6月中国中医科学院西苑医院肾病科门诊及住院IgAN患者36例为IgAN组,健康自愿者20例为健康对照组。除伴高血压患者服用降压药[血管紧张素转化酶抑制剂和(或)二氢吡啶类钙拮抗剂]外,IgAN组均只口服对证中药汤剂3个月,健康对照组不予任何干预措施。运用流式细胞仪检测IgAN组治疗前后Th1、Th2、CD4+CD25+Treg水平及患者尿红细胞数;运用免疫比浊法检测24h尿蛋白定量。结果与健康对照组比较,IgAN组CD4+CD25+Treg水平明显降低,差异有统计学意义(P<0.01);IgAN组中药治疗后Th1、尿红细胞数及24h尿蛋白定量均较治疗前明显降低,差异有统计学意义(均P<0.05)。结论中药治疗可减少IgAN患者的尿红细胞数及24h尿蛋白定量,其作用机制可能是中药治疗可下调Th1表达。
Objective To observe the effect of Chinese herbal therapy on T-lymphocyte subsets in patients with IgA nephropathy （IgAN）. Methods Totally 36 inpatients and outpatients at Department of Nephropathy, Xiyuan Hospital, China Academy of Chinese Medical Sciences, from June 2011 to June 2013 were recruited in the treatment group, while 20 volunteers were recruited as the healthy control group. Patients in the IgAN group only took Chinese herbal decoctions by syndrome typing for 3 months （except those accompanied with hypertension additionally took antihypertensive agents such as angiotensin-converting enzyme inhibitor and/or dihydropyridines calcium antagonist）. No intervention was performed in the healthy control group. The values of Thl, Th2, and CD4 ＋ CD25 ＋ Treg, and red blood cell number in urine were detected using flow cytometry before and after treatment. 24 h urine protein was detected using inmmunoturbidimetry. Results Compared with the healthy control group, the CD4 ＋ CD25 ＋ Treg level obviously decreased in the IgAN group, showing statistical difference （P 〈0.01 ）. In the IgAN group, Thl, 24 h urine protein, and urine red blood cell counts were obviously lower after treatment, showing statistical difference when compared with before treatment （all P 〈0.05）. Conclusion Chinese herbal therapy could reduce urine erythrocyte number and 24 h urine protein of IgAN patients, and downregulating Thl expression might be its mechanism.
出处
《中国中西医结合杂志》
CAS
CSCD
北大核心
2014年第7期786-789,共4页
Chinese Journal of Integrated Traditional and Western Medicine
基金
国家自然科学基金资助项目(No.C190402)
关键词
中药治疗
IGA肾病
T淋巴细胞
Chinese herbal therapy
IgA nephropathy
T lymphocyte
