摘要
目的:研究小檗碱脂质体对代谢综合征(MS)患者大网膜脂肪组织糖原合成酶激酶-3β(GSK-3β)及其磷酸化的调节作用。方法:采用不同浓度的小檗碱脂质体干预大网膜脂肪组织,通过葡萄糖氧化酶-过氧化物酶法测定培养上清液中葡萄糖浓度,Realtime PCR检测GSK-3β基因的表达,Western blot测定pGSK-3β、GSK-3β蛋白的表达。结果:与对照组比较,小檗碱原药及脂质体可降低上清液中葡萄糖浓度,显著下调GSK-3β基因的表达,使pGSK-3/GSK-3β蛋白比值显著升高,皆以1×10-6mol/L脂质体组调节作用最为明显。结论:小檗碱脂质体可显著改善脂肪组织的葡萄糖代谢,其机制可能与增加GSK-3β的磷酸化、使活化GSK-3β减少有关。
Objective:To investigate the expression of glycogen synthase kinase 3 beta(GSK-3β) phosphory lation of berberine hydrochloride liposome in omental adipose tissue of metabolic syndrome(MS). Methods:Omental adipose tissues were cultivated and interfered with berberine hydrochloride liposome. The glucose concent rations were measured by GOD-POD method. GSK-3βmRNA in adipose tissue was detected by realtime polymerase chain reaction(realtime-PCR). Phosphorylated form of GSK-3β was confirmed by Western blot. Results:The glucose concentrations in berberine and hydrochloride liposome were found to be lower than the control group. Berberine hydrochloride liposome treatment decreased GSK-3β expression dose-dependently,but increased pGSK-3β/GSK-3β production sharply. Conclusion:Berberine hydrochloride liposome can improve glucose metabolism of adipose tissues. The mechanism is possibly associated with the increase of GSK-3β phosphorylation.
出处
《山东中医药大学学报》
2014年第4期389-391,共3页
Journal of Shandong University of Traditional Chinese Medicine
基金
南京市卫生局医学科技发展重点项目(编号:ZKX09031)
关键词
代谢综合征
脂肪组织
小檗碱脂质体
糖原合成酶激酶-3Β
糖原合成酶激酶-3β磷酸化
metabolic syndrome adipose tissue berberine hydrochloride liposome glycogen synthase kinase-3 beta glycogen synthase kinase-3 beta phosphorylation