期刊文献+

小檗碱脂质体对代谢综合征患者大网膜脂肪组织GSK-3β磷酸化的调节作用

Effect of Berberine Hydrochloride Liposome on Expression of GSK-3β Phosphorylation in Omental Adipose Tissue of Metabolic Syndrome Patients
下载PDF
导出
摘要 目的:研究小檗碱脂质体对代谢综合征(MS)患者大网膜脂肪组织糖原合成酶激酶-3β(GSK-3β)及其磷酸化的调节作用。方法:采用不同浓度的小檗碱脂质体干预大网膜脂肪组织,通过葡萄糖氧化酶-过氧化物酶法测定培养上清液中葡萄糖浓度,Realtime PCR检测GSK-3β基因的表达,Western blot测定pGSK-3β、GSK-3β蛋白的表达。结果:与对照组比较,小檗碱原药及脂质体可降低上清液中葡萄糖浓度,显著下调GSK-3β基因的表达,使pGSK-3/GSK-3β蛋白比值显著升高,皆以1×10-6mol/L脂质体组调节作用最为明显。结论:小檗碱脂质体可显著改善脂肪组织的葡萄糖代谢,其机制可能与增加GSK-3β的磷酸化、使活化GSK-3β减少有关。 Objective:To investigate the expression of glycogen synthase kinase 3 beta(GSK-3β) phosphory lation of berberine hydrochloride liposome in omental adipose tissue of metabolic syndrome(MS). Methods:Omental adipose tissues were cultivated and interfered with berberine hydrochloride liposome. The glucose concent rations were measured by GOD-POD method. GSK-3βmRNA in adipose tissue was detected by realtime polymerase chain reaction(realtime-PCR). Phosphorylated form of GSK-3β was confirmed by Western blot. Results:The glucose concentrations in berberine and hydrochloride liposome were found to be lower than the control group. Berberine hydrochloride liposome treatment decreased GSK-3β expression dose-dependently,but increased pGSK-3β/GSK-3β production sharply. Conclusion:Berberine hydrochloride liposome can improve glucose metabolism of adipose tissues. The mechanism is possibly associated with the increase of GSK-3β phosphorylation.
出处 《山东中医药大学学报》 2014年第4期389-391,共3页 Journal of Shandong University of Traditional Chinese Medicine
基金 南京市卫生局医学科技发展重点项目(编号:ZKX09031)
关键词 代谢综合征 脂肪组织 小檗碱脂质体 糖原合成酶激酶-3Β 糖原合成酶激酶-3β磷酸化 metabolic syndrome adipose tissue berberine hydrochloride liposome glycogen synthase kinase-3 beta glycogen synthase kinase-3 beta phosphorylation
  • 相关文献

参考文献8

二级参考文献47

  • 1殷峻,陈名道,唐金凤,李凤英,周丽斌,杨颖,陈家伦.小檗碱对实验大鼠糖脂代谢的影响[J].中国糖尿病杂志,2004,12(3):215-218. 被引量:54
  • 2王健松,朱家壁,吕瑞勤,沈伟.肺靶向阿奇霉素脂质体的制备及其在小鼠体内的分布[J].药学学报,2005,40(3):274-278. 被引量:30
  • 3黄林清,徐传福,周世文,张诗平,姚丹凡.小檗碱抗肿瘤作用实验研究[J].中国药理学通报,1997,13(2):189-189. 被引量:25
  • 4陈其明 谢明智.小檗碱对正常小鼠血糖调节的影响[J].药学学报,1987,22(3):161-161. 被引量:29
  • 5倪艳霞 刘安张 等.黄连素治疗Ⅱ型糖尿病60例疗效观察及实验研究[J].中西医结合杂志,1988,8(12):711-711. 被引量:37
  • 6陈其明.黄连及小檗碱降血糖作用的研究[J].药学学报,1986,21(6):401-401. 被引量:63
  • 7Chawla A, Nguyen KD, Goh YP. Macrophage-mediated inflamma- tion in metabolic disease[ J]. Nat Rev Immunol, 2011,11 ( 11 ) :738-749. 被引量:1
  • 8Prins JB, O'Rahilly S. Regulation of adipose cell number in man [ J]. Clin Sci,1997,92( 1 ) :3-11. 被引量:1
  • 9Lee YS, Kim WS, Kim KH, et al. Berberine, a natural plant prod- uct, activates AMP-activated protein kinase with beneficial metabolic effects in diabetic and insulin-resistant states [ J ]. Diabetes, 2006,55 (8) : 2256-2264. 被引量:1
  • 10Chen QM, Xie MZ. Studies on the hypoglycemic effect of coptis chinensis and berberine [ J]. Yao Xue Xue Bao, 1986,21 (6) :401-406. 被引量:1

共引文献41

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部