摘要
目的:探讨片仔癀诱导骨肉瘤U2OS细胞凋亡与线粒体跨膜电位(Δψm)的关系。方法:采用不同浓度的片仔癀(0.8、1.2、1.6mg/mL)作用U2OS细胞后,Hoechst 33258染色镜下观察、Annexin V-FITC/PI双染流式细胞仪检测细胞凋亡情况;JC-1染色流式细胞仪检测线粒体跨膜电位;分光光度法检测Caspase-9、Caspase-3活性;Western blot检测Bcl-2、Bax蛋白的表达。结果:随剂量的增加,片仔癀可明显诱导骨肉瘤U2OS细胞凋亡、降低细胞线粒体膜电位,增加Caspase-9、Caspase-3活性,各干预组与阴性对照组比较均有明显差异(P<0.05,P<0.01),Western blot检测结果表明Bcl-2蛋白表达下降,而Bax蛋白表达上调。结论:片仔癀可降低细胞线粒体跨膜电位,改变线粒体通透性,促使骨肉瘤细胞凋亡。
Objective： To study the relationship in apoptosis and mitochondrion membrane potential （Δψm） of osteosarcoma U2OS cells induced by Pien Tze Huang. Methods： Human osteosarcoma U2OS cells were treated by different concentrations of Pien Tze Huang （0.8, 1.2, 1.6mg/mL）. The apoptosis was detected by Hoechst 33258 staining assay and flow cytometry. The changes of mitochondrial membrane potential were detected by JC-1 staining assay, and the activities of Caspase-9 and Caspase-3 were determined with spectrophotometry. Western blot was used to test the expression of Bcl-2 and Bax. Results： With the increase of the dose, Pien Tze Huang could induce the apoptosis of U2OS cells, decrease electric potential of mitochondrial membrane and increase the activities of Caspase-9 and Caspase-3 significantly. There were significant differences between treatment groups and the control group （P〈0.05, P〈0.01）. The result of Western blot showed that the expression of Bcl-2 decreased and that of Bax increased. Conclusion： Pien Tze Huang could decrease the electric potential of mitochondrial membrane, change the mitochondrial permeability and improve the apoptosis of osteosarcoma ceils.
出处
《中华中医药杂志》
CAS
CSCD
北大核心
2014年第8期2571-2574,共4页
China Journal of Traditional Chinese Medicine and Pharmacy
基金
国家自然科学基金项目(No.30901916
No.81373659)~~
关键词
片仔癀
骨肉瘤
线粒体膜电位
凋亡
Pien Tze Huang
Osteosarcoma
Mitochondrial membrane potential
Apoptosis
