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利用花药培养技术创制籼型两系不育材料

Generating Two-line Sterile Material of Indica Rice Using Anther Culture Technology
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摘要 以培矮64S、福龙S2、F1、H1为试验材料,研究不同播种期对F1代花药培养力的影响,H1群体自然加倍率,愈伤苗系(同一块愈伤组织分化的一丛绿苗,以下简称愈伤苗系)的农艺性状和H1群体基因变异性DNA检测等。结果表明:不同播种期对花药培养力的影响较大;H1群体的二倍体率为64.25%;该DH群体中,大部分愈伤苗系的农艺性状整齐一致,所考查的农艺性状中,除播始历期外,均存在双向超亲优势;利用SSR标记对H1群体中的全体单株进行检测,未发现基因突变或染色体结构变异的存在。 Applicability of the anther culture of F1 generation on Indiea rice as affected by the sowing date was studied. The natural doubling rate of H1 population, the agronomic traits of callus seedlings lines (i. e. , the green seedlings differentiated from a same callus), and the genetic variability detection on H1 population by DNA technology were investigated using Peiai 64S, S2, F1 and H1 generation for the experimentation. The results showed that (a) sowing date significantly affected the applicability of the anther cultivation; (b) the diploid natural doubling rate of Hi population was 64.25% (c) the agronomic traits of most callus seedlings lines were uniform; (d) there were bidirectional super parent advantages, except the perieds, between the seeding date and the heading stage; and, (e) the genetic mutation or chromosome structure variation was not detected in H1 population by SSR molecular marker.
出处 《福建农业学报》 CAS 2014年第6期515-518,共4页 Fujian Journal of Agricultural Sciences
基金 福建省区域重大科技项目(2010N016) 龙岩市科技计划项目(2012LY76)
关键词 水稻 两系不育系 材料创制 花药培养 分子标记 oryza sativa dual-purpose genic male sterile line material creation anther culture molecular marker
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