摘要
通过整体原位杂交和切片原位杂交技术系统全面地比较分析了斑马鱼胚胎四个发育时期(12、24、48 hpf和4 dpf)和成熟性腺中Sox9a和Sox9b基因的表达模式。结果显示,两个Sox9基因在脑和眼中持续表达,但在耳囊、头部软骨、胸鳍芽和体节中差异性表达。Sox9a特异性地在侧线、尾部神经嵴、原肾管和精巢支持细胞中表达,而Sox9b在卵巢的卵黄颗粒中大量表达。与已发表的研究结果相比,本研究更广泛地揭示了Sox9a和Sox9b的动态差异表达模式,且更清楚地显示了其在性腺中的表达特征。
Using whole mount and tissue section in situ hybridization techniques,the expression patterns of two Sox9 homologues genes,Sox9a and Sox9b,were comprehensively compared and analyzed in four stages of developing embryo（12,24,48 hpf and 4 dpf） and mature gonads of zebrafish（Danio rerio）.The two genes were persistently expressed in brain and eyes,but differentially expressed in otic vesicle,head cartilage,pectoral fin buds and somites.Distinct from high expression of Sox9b in the yolk granules of oocytes,Sox9a was specifically expressed in lateral line,tail neural crest,primary renal tubular and Sertoli cells in testis.Compared with other published results,the present data revealed a more extensive differential expression profile of two Sox9 genes in the developing embryo and gonads of zebrafish.
出处
《山东农业科学》
2014年第7期20-24,F0003,共6页
Shandong Agricultural Sciences
基金
上海市教委科研创新项目(13ZZ126)
上海海洋大学斑马鱼再生技术启动基金(A2400120007)资助
