摘要
为研究LFY、FLC及SOC1开花调控关键节点基因在白桦花时调控中的作用,从白桦转录组数据中获得了LFY和FLC的全长cDNA序列,分别命名为BpLFY和BpFLC。获得的BpLFY基因编码区长1215bp,编码405个氨基酸,分子量为45.3 kD。BpFLC基因cDNA编码区长627 bp,编码209个氨基酸,分子量为11.4 kD,这2个基因分别属于FLO-LFY和MADS超家族。对3个基因的启动子进行分析,发现3个基因启动子区均含有2个花粉特异表达的顺式作用元件。通过实时定量PCR对BpLFY、BpFLC及BpSOC1在白桦茎尖、叶片、雄花序和叶腋中表达模式的分析结果表明,BpSOC1和BpFLC在白桦各个组织部位均表达,而BpLFY只在雄花序和发育中的腋芽中表达,且在叶片中BpFLC可抑制BpSOC1的表达。
LFY,FLC and SOC1 genes are key node genes in flowering regulation network.To characterize their function in birch,complete cDNA sequences of LFY and FLC were obtained from transcriptome datas of Betula platyphylla,named BpLFY and BpLFY,respectively.BpLFY was 1 215 bp in length,encoding a protein of 405 a-mino acid residues with the molecular mass of 45 .3 kD.The length of BpLFY gene cDNA was 627 bp,encoding 209 amino acid residues with the molecular mass of 1 1.4 kD,the two genes belonged to FLO-LFY and MADS su-perfamily,respectively.Analysis of promoters of these genes showed that promoters of all these genes contained two cis-acting elements of pollen specific expression.In different tissues （shoot tip,leaf,male inflorescence and axilla-ry）,gene expression of BpLFY,BpFLC and BpSOC1 genes were investigated by using real-time quantitative RT-PCR.The results showed that BpSOC1 and BpFLC were expressed in various tissues of birch,however,BpLFY was expressed only in the male inflorescence and axillary,and BpFLC can inhibit BpSOC1 expression in the leaves.
出处
《西南林业大学学报（自然科学）》
CAS
2014年第4期20-25,共6页
Journal of Southwest Forestry University：Natural Sciences
基金
"十二五"农村领域国家科技计划课题(2013AA102704)资助
