摘要
介绍了耐酸耐高温α-淀粉酶在工业生产中具有经济、节能、方便操作等优势;并且概述了耐酸耐高温α-淀粉酶的菌种来源,其中耐酸性α-淀粉酶的菌种主要来源于芽孢杆菌、曲霉以及嗜热真菌;耐高温α-淀粉酶的菌种主要来源于凝结芽孢杆菌、枯草芽孢杆菌、嗜热芽孢杆菌、地衣芽孢杆菌和嗜热网络球杆菌等;本文还对耐酸耐高温α-淀粉酶的菌种选育技术方法包括物理方法、化学方法以及基因操作技术等进行了阐述。研究发现对耐酸耐高温α-淀粉酶的发酵工艺进行优化后,最高可以使酶活提高2.1倍;同时在对该淀粉酶的钙离子依赖性进行研究中,得出某些耐酸耐高温α-淀粉酶具有不依赖Ca2+的特性;最后预测人们将会运用基因工程等技术手段,不断地开发出各种特性的耐酸耐高温性α-淀粉酶。
The paper introduced the acid - resistant thermostable α - amylase with the advantages of economy, energy saving and easy to use in starch hydrolyzation. It also concluded the enzyme producing strains, mainly from Bacillus, Aspergillus and Thermomyces; The stains producing thermostable α - amylase were mainly from B. coagulans, B. subtilis, B. stearothermophilus, B. lieheniformis, Dictyoglomus thermophilum etc. ; The breeding methods of the acid - resistant thermostable α - amylase including physical, chemical and genetic engineering were also expounded. The study found that the enzyme activity was increased by 2. 1 times at most after optimizing in the fermentation process. The study on amylase calcium dependence revealed that some of the acid - resistant thermostable α - amylase were not dependent on ca2＋. Finally we forecast that researchers would make use of genetic engineering technology to develop various kinds of acid - resistant thermostable α- amylases for different purposes.
出处
《中国食品添加剂》
CAS
北大核心
2014年第4期171-176,共6页
China Food Additives
基金
国家科技支撑计划项目:耐酸耐高温-α淀粉酶的研发与应用项目编号:2013AA102101-1
