摘要
用离散偶极子近似(Discrete Dipole Approximation,DDA)对生物细胞的光散射特性进行数值模拟,分析讨论了基于生物细胞的相对折射率、散射截面、粒子直径等的光散射图像。分析表明,生物细胞的散射光强随着散射角度和相对折射率的增大而增大,散射的主要能量集中在10°散射角以内。然后设计了用于分析悬浮生物细胞的光散射实验装置,并以悬浮的牛肾细胞作为实验样品,进行实验研究。分析结果显示,模拟数据与实验数据符合较好。为进一步研究生物细胞散射特性以及无损检测提供一种理论和实验方法。
A non-destructive method was described for characterizing biological cells scattering. Firstly, the numerical simulation was performed to analyze the light scattering properties of biological cells based on the discrete dipole approximation （DDA）. Then the angular dependence of the scattering intensity related to the relative refractive index, and the particle diameter were discussed in detail. The analysis shows that the scattering intensity of the living cells increases with the scattering angle and relative refractive index, and the main scattering energy is concentrated in the scattering angle less than 10° . In addition, an experimental measurement on suspended bovine kidney cells was performed and the results was compared with the simulation. Finally, the angular distribution of scattering from the simulation and experiment matched well at the angle range of 5°to 52°. This result demonstrates that DDA can be used for further theoretical research and experimental methods for biological cells scattering characteristics.
出处
《红外与激光工程》
EI
CSCD
北大核心
2014年第7期2204-2208,共5页
Infrared and Laser Engineering
基金
广东省自然科学基金(9151064201000035)
光电信息与传感技术广东普通高校重点实验室开放研究基金(Gdo1201201)
关键词
离散偶极子近似(DDA)
生物细胞
非球形粒子
光散射
discrete dipole approximation （DDA）
biological ceils
non-spherical particles
light scattering
