摘要
目的:研究miR-342-3p对乳腺癌化疗敏感性的影响。方法:检测乳腺癌细胞株MCF-7、SKBr3和MDA-MB-231中miR-342-3p的表达。应用脂质体转染方法,转染hsa-miR-342-3p模拟物到低表达miR-342-3p的乳腺癌细胞株(mimic转染组),同时设立阴性对照(mim-NC转染组);转染miR-342-3p抑制物到高表达miR-342-3p的乳腺癌细胞株(inhibitor转染组),同时设立阴性对照(inhi-NC转染组)。mimic转染组、mim-NC转染组、inhibitor转染组和inhi-NC转染组细胞,分别加入浓度为2μmol/L的紫杉醇、顺铂及4μmol/L的阿霉素的进行培养,应用CCK8法检测药物作用48 h后细胞增殖率的变化。结果:以SKBr3为参照,miR-243-3p在MCF-7细胞中的相对表达倍数是126.000,在MDA-MB-231细胞中的相对表达倍数是0.017。mimic转染组细胞与紫杉醇、顺铂孵育48 h后,肿瘤细胞增殖率低于mim-NC转染组,差异有统计学意义(P<0.05),但与阿霉素孵育后细胞增殖率与mim-NC转染组差异无统计学意义(P>0.05);inhibitor转染组细胞与紫杉醇、顺铂和阿霉素孵育48 h后,肿瘤细胞的增殖率高于inhi-NC转染组,差异有统计学意义(P<0.05)。结论:miR-342-3p能调控乳腺癌细胞株MDA-MB-231、MCF-7对紫杉醇和顺铂的化疗敏感性,但提高miR-342-3p表达不能增加MDA-MB-231细胞对阿霉素的化疗敏感性,降低miR-342-3p的表达却可以减弱MCF-7细胞对阿霉素的化疗敏感性。
Objective：To explore the effect of miR-342-3p on chemotherapy sensitivity of breast cancer cells.Methods：The expression levels of miR-342-3p were detected in breast cancer cell lines MCF-7,SKBr3 and MDA-MB-231.By using lipofectamine,the hsa-miR-342-3p mimic was transfected into breast cancer cell lines,which were of the lowest expression of miR-342-3p cell lines（the mimic group）.The mim-NC was performed as the negative control group.Furthermore,the miR-342-3p inhibitor was transfected into breast cancer cell lines of the highest expression of miR-342-3p,the inhi-NC was performed as the negative control group.Cells from four different groups（the mimic,mim-NC,inhibitor and inhi-NC groups） were treated with 2 μmol/L paclitaxel,2 μmol/L cisplatin and 4 μmol/L doxorubicine for 48 hours,respectively.CCK8 assay was used for detection of cell proliferation.Results：Compared with the expression level of miR-243-3p in SKBr3,the level of miR-243-3p in MCF-7 cells was significantly increased（126.000 fold change）,but it was decreased（0.017 fold change） in MDA-MB-231 cell lines.The rates of cell proliferation in the mimic group after treatment with paclitaxel and cisplatin for 48 hours were significantly lower than those in the mim-NC group,respectively（P〈 0.05,respectively）.However,the cell proliferation rates in the mimic group and the mim-NC group after treatment with doxorubicine for 48 hours had no significant difference（P〈 0.05）.The cell proliferation rates in the inhibitor group were significantly higher than those in the inhi-NC groups after treatment with paclitaxel,cisplatin and doxorubicine for 48 hours,respectively（P〈 0.05,respectively）.Conclusion：miR-342-3p may play a key role in the regulation of chemotherapy sensitivity to paclitaxel and cisplatin in breast cancer cell lines MDA-MB-231 and MCF-7.Up-regulation of miR-342-3p expression could not increase the chemotherapy sensitivity to doxorubicine,but down-regulation of miR-342-3p expression may weaken the chemotherapy
出处
《南京医科大学学报（自然科学版）》
CAS
CSCD
北大核心
2014年第6期716-720,共5页
Journal of Nanjing Medical University(Natural Sciences)
基金
无锡市卫生局科研项目计划资助(ML201307)
