摘要
目的：评价不同剂量右美托咪定预先给药对大鼠布比卡因心脏毒性的影响。方法健康雄性 SD 大鼠48只,体重250-300 g,采用随机数字表法,将其随机均分为四组：生理盐水对照组（C 组）、右美托咪定5μg/kg 组（D5组）、右美托咪定10μg/kg 组（D10组）和右美托咪定15μg/kg 组（D15组）。以肢体Ⅱ导联监测 ECG,股动脉置管监测 MAP,股静脉置管给药。D5、D10、D15组分别于布比卡因给药前15 min 时经股静脉分别泵注右美托咪定5、10和15μg/kg,C 组给予等容量生理盐水。四组大鼠均泵注0.75%布比卡因2 mg·kg-1·min-1。分别记录大鼠发生抽搐、心律失常及心脏停搏的时间和布比卡因的用量,并测定心肌组织布比卡因含量。结果与C 组比较, D5、D10和 D15组发生抽搐、心律失常和心脏停搏的时间明显延长（P 〈0.05）,布比卡因的用量及心肌布比卡因含量明显增加（P 〈0.05）；与 D5组比较,D10和 D15组发生抽搐、心律失常和心脏停搏的时间明显延长,布比卡因的用量及心肌布比卡因含量明显增加（P 〈0.05）；D10与 D15组发生抽搐、心律失常和心脏停搏的时间、布比卡因的用量及心肌布比卡因含量差异无统计学意义。结论静脉内预先给予右美托咪定可明显提高布比卡因中毒剂量阈值,延迟布比卡因心脏毒性的发生时间,减轻大鼠布比卡因心脏毒性反应,且在一定剂量范围内呈剂量依赖性。
Objective To investigate the effects of different doses of dexmedetomidine pretreat-ment on cardiac toxicity of bupivacaine in rats.Methods Forty eight adult male SD rats weighing 250-300 g were randomly divided into 4 groups （n=12）：saline control group （group C）,dexmedetomi-dine 5 μg/kg group（group D5）,dexmedetomidine 10 μg/kg group（group D10）and dexmedetomidine 1 5 μg/kg group（group D1 5 ）.A Ⅱ-lead electrocardiogram（ECG）was continuously monitored,the femoral artery was cannulated for direct measurement of MAP and the femoral vein was cannulated for infusion of drugs.Groups D5,D10 and D1 5 were received infusion of dexmedetomidine 5,10 and 1 5μg/kg respectively 1 5 minutes before administration of bupivacaine,while the equal volume of saline was given in group C,then all rats received infusion 0.75% bupivacaine at the rate of 2 mg·kg-1· min-1 until asystole occurred.The doses of bupivacaine and the times of bupivacaine-induced convul-sions,arrhythmia and asystole were recorded respectively,and the myocardial concentration of bupiv-acaine was observed.Results Compared with group C,the doses of bupivacaine and the times of bupivacaine-induced convulsions,arrhythmia and asystole were all increased in groups D5,D10 and D1 5 （P 〈0.05）.Compared with group D5,the above parameters were increased in groups D10 and D1 5 （P 〈0.05 ）.There was no statistical significance of the above parameters between groups D10 and group D1 5.Conclusion Dexmedetomidine pretreatment can raise the threshold toxic dose of bupi-vacaine,delay the time of occurrence of cardiotoxicity of bupivacaine,so that to prevent the cardiac toxicities of bupivacaine in rats,and it produces a dose-dependent protective effect within a certain dose range.
出处
《临床麻醉学杂志》
CAS
CSCD
北大核心
2014年第7期689-692,共4页
Journal of Clinical Anesthesiology
