摘要
运用MTT法、细胞形态学观察和细胞克隆实验观察一个新型的呫吨酮并吡啶衍生物(XP-16)对人鼻咽癌CNE细胞的体外抗肿瘤效应;运用Hoechest33258和PI双染、流式细胞仪检测、荧光光度法、RT-PCR等手段研究其可能的作用机制。结果表明,该化合物能剂量和时间依赖性地抑制CNE细胞增殖;XP-16作用于CNE细胞24 h后,CNE细胞出现染色质聚集、核碎裂等典型的凋亡形态学改变,且随化合物剂量的增加,CNE细胞凋亡百分率逐渐增大;CNE细胞阻滞于G2-M和S期;并且引起CNE细胞线粒体膜电位降低、Bad和MT-1A的mRNA表达增加。XP-16具有诱导CNE细胞凋亡作用,其作用机制可能与其降低线粒体膜电位、上调MT-1A表达有关。
MTT assay, morphological examination and colonial assay were applied to evaluate the anti-proliferative effect of a new xanthono-pyridine derivative （XP-16） on human nasopharyngeal carcinomaCNE cells Hoechest 33258/PI d ouble staining,also employed to investigate its possible actionflow cytometry, spectrofluorimetry and RT-PCR weremechanism. The experiment results showed that XP-16could inhibit the proliferation of CNE cells in dose- and time-dependent manner. Typical apoptotic mor-phology such as chromatin aggregation and nuclear fragmentation was observed in XP-16 treated CNEcells for 24 h in a dose-dependent manner. After treated with XP-16, CNE cells were blocked in G2-Mand S phases, mitochondria membrane potential of the cells was decreased, relative Bad and MT-1AmRNA level was up-regulated. The apoptosis of CNE cells inducing by XP-16 might be associated withdecreasing mitochondria membrane potential and up-regulating MT-1A.
出处
《广西师范大学学报（自然科学版）》
CAS
北大核心
2014年第2期95-100,共6页
Journal of Guangxi Normal University:Natural Science Edition
基金
国家自然科学基金资助项目(21002015)
广西自然科学基金资助项目(2010GXNSFB013013
0639030)
关键词
呫吨酮
抗肿瘤
人鼻咽癌CNE细胞
凋亡
xanthone
antitumor
human nasopharyngeal carcinoma cell line CNE
apoptosis
