摘要
目的以总黄酮醇苷、萜类内酯的综合转移率为指标,确定银杏叶最佳提取工艺。方法采用正交设计方法,系统考察乙醇浓度、料液比、提取时间3个因素对银杏叶提取工艺的影响,利用高效液相色谱法测定总黄酮醇苷、萜类内酯的含量,计算转移率。结果最佳提取工艺条件为:采用85%乙醇加热回流提取3次,第1次加5倍量(V/W)溶剂提取3 h,后2次分别加入3倍量(V/W)溶剂提取2 h。结论本工艺操作简单、合理、节能、高效,适用于工业化生产。
Objective To determine the best extraction process of ginkgo leaves with the total transfer rate of total flavonol glycosides and terpene lactones as the index.Methods The effects of ethanol concentration, solid-liquid ratio and extraction time on extraction process were investigated by orthogonal design method, and the contents of total flavonol glycosides and terpene lactones were detected by HPLC to calculate transfer rate.Results The optimum extraction conditions were as follows：85% ethanol refluxing and extracting for three times;the first time extracting with five-fold amount of solvent （V/W） for 3 hours;the last two times with three-fold solvent （V/W） for 2 hours.Conclusion This extraction process has the advantages of simplicity of operator, reason, energy conservation, high efficiency, and is suitable for industrial production.
出处
《中国中医药信息杂志》
CAS
CSCD
2014年第8期78-80,共3页
Chinese Journal of Information on Traditional Chinese Medicine
基金
浙江省科技计划项目(2009E10009)
浙江省企业研究院建设计划(2010E90011)
关键词
银杏叶
总黄酮醇苷
萜类内酯
转移率
正交试验
ginkgo leaves
total flavonol glycosides
terpene lactones
transfer rate
orthogonal experiment
